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Product Info Summary
| SKU: | EK1207 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PSP94/MSMB ELISA Kit PicoKine®
View all PSP94/MSMB ELISA kits
SKU/Catalog Number
EK1207
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PSP94 ELISA Kit PicoKine® (96 Tests). Quantitate Human MSMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PSP94/MSMB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1207)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S21-I114
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1207 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1207 is reactive to MSMB in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1207 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PSP94/MSMB
Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11.23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory (tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1207-CAP | Anti-Human MSMB Pre-coated 96-well strip microplate | 1 |
| EK1207-ST | Human MSMB Standard | 2 vials, 10 ng/tube |
| EK1207-DA | Human MSMB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.084 | 0.225 | 0.323 | 0.462 | 0.792 | 1.291 | 1.970 | 2.548 |
Data Example Images
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Human PSP94 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PSP94/MSMB, Dilution ratio of 1:1, concentration in serum and plasma is around 2 ng/ml..
Some articles we found to cite concentrations of PSP94/MSMB in samples: 17062675 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PSP94/MSMB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 160 | 628 | 1438 | 148 | 589 | 1345 |
| Standard deviation | 9.44 | 49.61 | 113.6 | 8.73 | 53.59 | 130.46 |
| CV (%) | 7.9% | 7.9% | 7.9% | 5.9% | 9.1% | 9.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PSP94/MSMB in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 160 | 156 | 160 | 151 | 156 | 3.69 | 2.3% |
| Sample 2 | 628 | 662 | 671 | 720 | 670 | 32.89 | 4.9% |
| Sample 3 | 1438 | 1274 | 1320 | 1318 | 1337 | 60.86 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For MSMB (Source: Uniprot.Org, NCBI)
Gene Name
MSMB
Full Name
Beta-microseminoprotein
Weight
12865 MW
Superfamily
beta-microseminoprotein family
Alternative Names
Beta-microseminoprotein;Immunoglobulin-binding factor;IGBF;PN44;Prostate secreted seminal plasma protein;Prostate secretory protein of 94 amino acids;PSP-94;PSP94;Seminal plasma beta-inhibin;MSMB;PRSP; MSMB HPC13, IGBF, MSP, MSPB, PN44, PRPS, PSP, PSP-94, PSP57, PSP94 microseminoprotein beta beta-microseminoprotein|immunoglobulin binding factor|prostate secreted seminal plasma protein|prostate secretory protein of 94 amino acids|seminal plasma beta-inhibin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MSMB, check out the MSMB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MSMB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PSP94/MSMB ELISA Kit PicoKine® (EK1207)
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Customer Q&As
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7 Customer Q&As for Human PSP94/MSMB ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated PSP94 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PSP94 antigen?
Verified Customer
Verified customer
Asked: 2020-10-24
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-10-24
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-08-07
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-08-07
Question
Q: is it okay to use citrate plasma as samples in Human PSP94 Picokine® ELISA Kit (Catalog # EK1207)?
Verified Customer
Verified customer
Asked: 2020-07-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of PSP94 causing disruption of its protein structure. PSP94 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the PSP94 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-07-17
Question
Q: which procedure should I follow in order to thaw whole blood sample for PSP94 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-01-05
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PSP94 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-01-05
Question
Q: What is the optimal O.D. value for PSP94 ELISA kit? I used your PSP94 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain PSP94 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PSP94 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of PSP94 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-06-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis PSP94 using the EK1207 Human PSP94 Picokine® ELISA Kit?
K. Nelson
Verified customer
Asked: 2016-08-27
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2016-08-27
Question
Q: can you recommend the dilution ratio of serum samples for detection of PSP94 in Human cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2016-02-12
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PSP94 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-02-12
