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Key=image_label, value=Western blot analysis of Apolipoprotein E expression in mouse spleen extract (lane 1) and mouse kidney extract (lane 2). Apolipoprotein E at 36KD was detected using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody (Catalog # PB9
Key=small_image_label, value=Western blot analysis of Apolipoprotein E expression in mouse spleen extract (lane 1) and mouse kidney extract (lane 2). Apolipoprotein E at 36KD was detected using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody (Catalog # PB9
Key=thumbnail_label, value=Western blot analysis of Apolipoprotein E expression in mouse spleen extract (lane 1) and mouse kidney extract (lane 2). Apolipoprotein E at 36KD was detected using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody (Catalog # PB9
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Key=protein_name, value=Apolipoprotein E
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Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
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Key=description, value=Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband® catalog # PB9986. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband® catalog # PB9986. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Apolipoprotein E/APOE Antibody Picoband®
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Key=gene_name, value=Apoe
Key=synonyms, value=Apolipoprotein E;Apo-E;Apoe;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Immunofluorescence, 5μg/ml, Mouse, Rat

Key=subcellular_localization, value= Secreted .
Key=tissue_specificity, value= Secreted in plasma.
Key=protein_function, value= Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. .
Key=application_notes, value=Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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Key=immunogen, value=E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=36 kDa
Key=custom_attribute_2, value=WB: mouse spleen tissue, mouse kidney tissue
IHC: mouse liver tissue, rat liver tissue
ICC/IF: HEPA1-6 cell
IF: mouse liver tissue, rat liver tissue
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Anti-Apolipoprotein E/APOE Antibody Picoband®

APOE antibody

Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband® catalog # PB9986. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9986
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-Apolipoprotein E/APOE Antibody Picoband®

View all APOE Antibodies

SKU/Catalog Number

PB9986

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband® catalog # PB9986. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apolipoprotein E/APOE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9986)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9986 is reactive to Apoe in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

36 kDa

Calculated molecular weight

35867 MW

Background of APOE

APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9986 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Immunofluorescence, 5μg/ml, Mouse, Rat

Positive Control

WB: mouse spleen tissue, mouse kidney tissue
IHC: mouse liver tissue, rat liver tissue
ICC/IF: HEPA1-6 cell
IF: mouse liver tissue, rat liver tissue

Validation Images & Assay Conditions

Gene/Protein Information For Apoe (Source: Uniprot.org, NCBI)

Gene Name

Apoe

Full Name

Apolipoprotein E

Weight

35867 MW

Superfamily

apolipoprotein A1/A4/E family

Alternative Names

Apolipoprotein E;Apo-E;Apoe; APOE AD2, APO-E, ApoE4, LDLCQ5, LPG apolipoprotein E apolipoprotein E|apolipoprotein E3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Apoe, check out the Apoe Infographic

Apoe infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Apoe: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-Apolipoprotein E/APOE Antibody Picoband®

Question

Would anti-Apolipoprotein E/APOE antibody PB9986 work for IHC with eye?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

According to the expression profile of eye, APOE is highly expressed in eye. So, it is likely that anti-Apolipoprotein E/APOE antibody PB9986 will work for IHC with eye.

Boster Scientific Support

Answered: 2020-03-16

Question

We are interested in using your anti-Apolipoprotein E/APOE antibody for fatty acid homeostasis studies. Has this antibody been tested with western blotting on mouse liver? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

We appreciate your inquiry. This PB9986 anti-Apolipoprotein E/APOE antibody is tested on mouse liver. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-09

Question

We need to test anti-Apolipoprotein E/APOE antibody PB9986 on rat eye for research purposes, then I may be interested in using anti-Apolipoprotein E/APOE antibody PB9986 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The products we sell, including anti-Apolipoprotein E/APOE antibody PB9986, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-04

Question

Is this PB9986 anti-Apolipoprotein E/APOE antibody reactive to the isotypes of APOE?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

The immunogen of PB9986 anti-Apolipoprotein E/APOE antibody is E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-28

Question

Is a blocking peptide available for product anti-Apolipoprotein E/APOE antibody (PB9986)?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

We do provide the blocking peptide for product anti-Apolipoprotein E/APOE antibody (PB9986). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-26

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-Apolipoprotein E/APOE antibody PB9986. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-11-26

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-26

Question

We have been able to see staining in mouse eye. Are there any suggestions? Is anti-Apolipoprotein E/APOE antibody supposed to stain eye positively?

Verified Customer

Verified customer

Asked: 2018-09-10

Answer

From what I have seen in literature eye does express APOE. From what I have seen in Uniprot.org, APOE is expressed in left adrenal gland, cerebellum, eye, blood, cerebrospinal fluid, liver, among other tissues. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-09-10

Question

See below the WB image, lot number and protocol we used for eye using anti-Apolipoprotein E/APOE antibody PB9986. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-07-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-02

Question

We purchased anti-Apolipoprotein E/APOE antibody for IHC on eye in a previous project. I am using rat, and We are going to use the antibody for WB next. I am looking for examining eye as well as left adrenal gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

N. Roberts

Verified customer

Asked: 2018-06-01

Answer

I looked at the website and datasheets of our anti-Apolipoprotein E/APOE antibody and it seems that PB9986 has been validated on rat in both IHC and WB. Thus PB9986 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-06-01

Question

We were satisfied with the WB result of your anti-Apolipoprotein E/APOE antibody. However we have observed positive staining in eye secreted. using this antibody. Is that expected? Could you tell me where is APOE supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

Based on literature, eye does express APOE. Generally APOE expresses in secreted. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2017-09-21

Question

Does PB9986 anti-Apolipoprotein E/APOE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Krishna

Verified customer

Asked: 2017-03-14

Answer

It shows on the product datasheet, PB9986 anti-Apolipoprotein E/APOE antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-03-14

Question

Do you have a BSA free version of anti-Apolipoprotein E/APOE antibody PB9986 available?

C. Carter

Verified customer

Asked: 2016-11-21

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Apolipoprotein E/APOE antibody PB9986 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-11-21

Question

I see that the anti-Apolipoprotein E/APOE antibody PB9986 works with IHC, what is the protocol used to produce the result images on the product page?

Z. Singh

Verified customer

Asked: 2016-05-04

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-05-04

Question

I was wanting to use your anti-Apolipoprotein E/APOE antibody for IHC for rat eye on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat eye identification?

T. Walker

Verified customer

Asked: 2014-07-01

Answer

You can see on the product datasheet, PB9986 anti-Apolipoprotein E/APOE antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat eye in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-07-01

Question

I have a question about product PB9986, anti-Apolipoprotein E/APOE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

H. Taylor

Verified customer

Asked: 2013-06-17

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9986 anti-Apolipoprotein E/APOE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-06-17

Question

We are currently using anti-Apolipoprotein E/APOE antibody PB9986 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on feline tissues as well?

A. Baker

Verified customer

Asked: 2013-03-05

Answer

The anti-Apolipoprotein E/APOE antibody (PB9986) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-03-05

Order DetailsPrice
PB9986

100μg

$370
PB9986-10ug

10μg sample (liquid)

$99
PB9986-Biotin

100 μg Biotin conjugated

$570
PB9986-Cy3

100 μg Cy3 conjugated

$570
PB9986-Dylight488

100 μg Dylight488 conjugated

$570
PB9986-Dylight550

100 μg Dylight550 conjugated

$570
PB9986-Dylight594

100 μg Dylight594 conjugated

$570
PB9986-FITC

100 μg FITC conjugated

$570
PB9986-HRP

100 μg HRP conjugated

$570
PB9986-APC

100 μg APC conjugated

$670
PB9986-PE

100 μg PE conjugated

$670
PB9986-iFluor647

100 μg iFluor647 conjugated

$670
PB9986-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9986
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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