Product Info Summary
SKU: | PB9986 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Apolipoprotein E/APOE Antibody Picoband®
SKU/Catalog Number
PB9986
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband® catalog # PB9986. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apolipoprotein E/APOE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9986)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9986 is reactive to Apoe in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
35867 MW
Background of APOE
APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9986 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Immunofluorescence, 5μg/ml, Mouse, Rat
Positive Control
WB: mouse spleen tissue, mouse kidney tissue
IHC: mouse liver tissue, rat liver tissue
ICC/IF: HEPA1-6 cell
IF: mouse liver tissue, rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9986).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein E antigen affinity purified polyclonal antibody (Catalog # PB9986) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein E at approximately 36 kDa. The expected band size for Apolipoprotein E is at 36 kDa.
Click image to see more details
Figure 2. IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9986).
Apolipoprotein E was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Apolipoprotein E Antibody (PB9986) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9986).
Apolipoprotein E was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Apolipoprotein E Antibody (PB9986) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (PB9986).
Apolipoprotein E/APOE was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Apolipoprotein E/APOE Antibody (PB9986) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (PB9986).
Apolipoprotein E/APOE was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Apolipoprotein E/APOE Antibody (PB9986) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (PB9986).
Apolipoprotein E/APOE was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Apolipoprotein E/APOE Antibody (PB9986) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Apoe (Source: Uniprot.org, NCBI)
Gene Name
Apoe
Full Name
Apolipoprotein E
Weight
35867 MW
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
Apolipoprotein E;Apo-E;Apoe; APOE AD2, APO-E, ApoE4, LDLCQ5, LPG apolipoprotein E apolipoprotein E|apolipoprotein E3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Apoe, check out the Apoe Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Apoe: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Apolipoprotein E/APOE Antibody Picoband® (PB9986)
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No publications found for PB9986
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Customer Q&As
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16 Customer Q&As for Anti-Apolipoprotein E/APOE Antibody Picoband®
Question
Would anti-Apolipoprotein E/APOE antibody PB9986 work for IHC with eye?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
According to the expression profile of eye, APOE is highly expressed in eye. So, it is likely that anti-Apolipoprotein E/APOE antibody PB9986 will work for IHC with eye.
Boster Scientific Support
Answered: 2020-03-16
Question
We are interested in using your anti-Apolipoprotein E/APOE antibody for fatty acid homeostasis studies. Has this antibody been tested with western blotting on mouse liver? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We appreciate your inquiry. This PB9986 anti-Apolipoprotein E/APOE antibody is tested on mouse liver. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-09
Question
We need to test anti-Apolipoprotein E/APOE antibody PB9986 on rat eye for research purposes, then I may be interested in using anti-Apolipoprotein E/APOE antibody PB9986 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The products we sell, including anti-Apolipoprotein E/APOE antibody PB9986, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-04
Question
Is this PB9986 anti-Apolipoprotein E/APOE antibody reactive to the isotypes of APOE?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The immunogen of PB9986 anti-Apolipoprotein E/APOE antibody is E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-28
Question
Is a blocking peptide available for product anti-Apolipoprotein E/APOE antibody (PB9986)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We do provide the blocking peptide for product anti-Apolipoprotein E/APOE antibody (PB9986). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-Apolipoprotein E/APOE antibody PB9986. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-26
Question
We have been able to see staining in mouse eye. Are there any suggestions? Is anti-Apolipoprotein E/APOE antibody supposed to stain eye positively?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
From what I have seen in literature eye does express APOE. From what I have seen in Uniprot.org, APOE is expressed in left adrenal gland, cerebellum, eye, blood, cerebrospinal fluid, liver, among other tissues. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-09-10
Question
See below the WB image, lot number and protocol we used for eye using anti-Apolipoprotein E/APOE antibody PB9986. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-02
Question
We purchased anti-Apolipoprotein E/APOE antibody for IHC on eye in a previous project. I am using rat, and We are going to use the antibody for WB next. I am looking for examining eye as well as left adrenal gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
N. Roberts
Verified customer
Asked: 2018-06-01
Answer
I looked at the website and datasheets of our anti-Apolipoprotein E/APOE antibody and it seems that PB9986 has been validated on rat in both IHC and WB. Thus PB9986 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-01
Question
We were satisfied with the WB result of your anti-Apolipoprotein E/APOE antibody. However we have observed positive staining in eye secreted. using this antibody. Is that expected? Could you tell me where is APOE supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
Based on literature, eye does express APOE. Generally APOE expresses in secreted. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-09-21
Question
Does PB9986 anti-Apolipoprotein E/APOE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Krishna
Verified customer
Asked: 2017-03-14
Answer
It shows on the product datasheet, PB9986 anti-Apolipoprotein E/APOE antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-03-14
Question
Do you have a BSA free version of anti-Apolipoprotein E/APOE antibody PB9986 available?
C. Carter
Verified customer
Asked: 2016-11-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Apolipoprotein E/APOE antibody PB9986 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-11-21
Question
I see that the anti-Apolipoprotein E/APOE antibody PB9986 works with IHC, what is the protocol used to produce the result images on the product page?
Z. Singh
Verified customer
Asked: 2016-05-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-04
Question
I was wanting to use your anti-Apolipoprotein E/APOE antibody for IHC for rat eye on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat eye identification?
T. Walker
Verified customer
Asked: 2014-07-01
Answer
You can see on the product datasheet, PB9986 anti-Apolipoprotein E/APOE antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-01
Question
I have a question about product PB9986, anti-Apolipoprotein E/APOE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Taylor
Verified customer
Asked: 2013-06-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9986 anti-Apolipoprotein E/APOE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-06-17
Question
We are currently using anti-Apolipoprotein E/APOE antibody PB9986 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on feline tissues as well?
A. Baker
Verified customer
Asked: 2013-03-05
Answer
The anti-Apolipoprotein E/APOE antibody (PB9986) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-05