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Product Info Summary
| SKU: | M00015 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Apolipoprotein E APOE Monoclonal Antibody
SKU/Catalog Number
M00015
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Apolipoprotein E APOE Monoclonal Antibody catalog # M00015. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apolipoprotein E APOE Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00015)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AbA15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Apolipoprotein E
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00015 is reactive to APOE in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00015 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human HepG2 whole cell, human K562 whole cell
IHC: human kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (M00015).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein E antigen affinity purified monoclonal antibody (Catalog # M00015) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein E at approximately 36 kDa. The expected band size for Apolipoprotein E is at 36 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kidney, using Apolipoprotein E Antibody.
Protein Target Info & Infographic
Gene/Protein Information For APOE (Source: Uniprot.org, NCBI)
Gene Name
APOE
Full Name
Apolipoprotein E
Weight
88068 MW
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; APOE AD2, APO-E, ApoE4, LDLCQ5, LPG apolipoprotein E apolipoprotein E|apolipoprotein E3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APOE, check out the APOE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APOE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Apolipoprotein E APOE Monoclonal Antibody (M00015)
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Customer Q&As
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15 Customer Q&As for Anti-Apolipoprotein E APOE Monoclonal Antibody
Question
I was wanting to use your anti-Apolipoprotein E Monoclonal antibody for IP for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
It shows on the product datasheet, M00015 anti-Apolipoprotein E Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-01
Question
We have been able to see staining in human cerebrospinal fluid. Do you have any suggestions? Is anti-Apolipoprotein E Monoclonal antibody supposed to stain cerebrospinal fluid positively?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Based on literature cerebrospinal fluid does express APOE. Based on Uniprot.org, APOE is expressed in left adrenal gland, cerebellum, eye, blood, cerebrospinal fluid, liver, among other tissues. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-04-22
Question
My question regarding product M00015, anti-Apolipoprotein E Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00015 anti-Apolipoprotein E Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-27
Question
We are currently using anti-Apolipoprotein E Monoclonal antibody M00015 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
J. Li
Verified customer
Asked: 2020-03-26
Answer
The anti-Apolipoprotein E Monoclonal antibody (M00015) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-26
Question
Is there a BSA free version of anti-Apolipoprotein E Monoclonal antibody M00015 available?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Apolipoprotein E Monoclonal antibody M00015 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Apolipoprotein E Monoclonal antibody M00015. Let me know if you need anything else.
S. Roberts
Verified customer
Asked: 2019-12-11
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
Is this M00015 anti-Apolipoprotein E Monoclonal antibody reactive to the isotypes of APOE?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of M00015 anti-Apolipoprotein E Monoclonal antibody is A synthesized peptide derived from human Apolipoprotein E. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
Our lab used your anti-Apolipoprotein E Monoclonal antibody for ICC on left adrenal gland a few months ago. I am using mouse, and We intend to use the antibody for WB next. I am interested in examining left adrenal gland as well as eye in our next experiment. Could give a recommendation on which antibody would work the best for WB?
C. Jones
Verified customer
Asked: 2019-07-08
Answer
I have checked the website and datasheets of our anti-Apolipoprotein E Monoclonal antibody and I see that M00015 has been validated on mouse in both ICC and WB. Thus M00015 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-08
Question
We were well pleased with the WB result of your anti-Apolipoprotein E Monoclonal antibody. However we have been able to see positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is APOE supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
Based on literature, liver does express APOE. Generally APOE expresses in secreted. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-08-23
Question
I see that the anti-Apolipoprotein E Monoclonal antibody M00015 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-23
Question
Is a blocking peptide available for product anti-Apolipoprotein E Monoclonal antibody (M00015)?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
We do provide the blocking peptide for product anti-Apolipoprotein E Monoclonal antibody (M00015). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-16
Question
Will M00015 anti-Apolipoprotein E Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
You can see on the product datasheet, M00015 anti-Apolipoprotein E Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-13
Question
Our lab want to know about to test anti-Apolipoprotein E Monoclonal antibody M00015 on mouse liver for research purposes, then I may be interested in using anti-Apolipoprotein E Monoclonal antibody M00015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Edwards
Verified customer
Asked: 2017-03-24
Answer
The products we sell, including anti-Apolipoprotein E Monoclonal antibody M00015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-24
Question
Will anti-Apolipoprotein E Monoclonal antibody M00015 work for IP with liver?
T. Carter
Verified customer
Asked: 2013-10-07
Answer
According to the expression profile of liver, APOE is highly expressed in liver. So, it is likely that anti-Apolipoprotein E Monoclonal antibody M00015 will work for IP with liver.
Boster Scientific Support
Answered: 2013-10-07
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Apolipoprotein E Monoclonal antibody M00015. Please let me know if you require anything else.
J. Yang
Verified customer
Asked: 2013-04-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-22
