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Product Info Summary
| SKU: | A00015-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-APO-E Antibody
SKU/Catalog Number
A00015-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APO-E Antibody (Catalog # A00015-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
APO-E antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-APO-E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00015-1)
Host
Rabbit
Contents
APO-E Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
APO-E antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E. The immunogen is located within amino acids 220 - 270 of APO-E.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00015-1 is reactive to APOE in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
36154 MW
Background of APOE
Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E (APO-E), a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Defects in APO-E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00015-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of APO-E inhuman liver tissue with APO-E antibody at 2.5 μg/ml.
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Immunofluorescence of APO E in mouse brain tissue with APO E Antibody at 20 μg/mL.
Green: APO E antibody (A00015-1)
Red: Phylloidin staining
Blue: DAPI staining
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Immunohistochemistry of APO E in mouse brain tissue with APO E Antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For APOE (Source: Uniprot.org, NCBI)
Gene Name
APOE
Full Name
Apolipoprotein E
Weight
36154 MW
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
AD2, LPG, LDLCQ5, Apolipoprotein E, Apo-E APOE AD2, APO-E, ApoE4, LDLCQ5, LPG apolipoprotein E apolipoprotein E|apolipoprotein E3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APOE, check out the APOE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APOE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APO-E Antibody (A00015-1)
Hello CJ!
A00015-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xue Y,Huang S,Huang J,Li S,Zhang C,Zhou X.Identification of Apolipoprotein E as a Potential Diagnostic Biomarker of Nasopharyngeal Carcinoma.Cancer Manag Res.2020 Sep 24;12:8943-8950.doi:10.2147/CMAR. S239479.PMID:33061590;PMCID:PMC7522425.
Species: Human
A00015-1 usage in article: APP:WB, SAMPLE:NPC TISSUES, DILUTION:NA
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