Product Info Summary
SKU: | A30987 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Goat |
Application: | ELISA, WB |
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Product info
Product Name
Anti-AP2A ap2a1-1 Antibody
View all alpha Adaptin Antibodies
SKU/Catalog Number
A30987
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-AP2A ap2a1-1 Antibody (Catalog # A30987). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-AP2A ap2a1-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30987)
Host
Goat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of Human AP2A1 and AP2A2 proteins.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30987 is reactive to AP2A1 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
10982 MW
Background of alpha Adaptin
The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit. The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30987 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:500 - 1:2,000
Positive Control
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AP2A using anti-AP2A antibody (A30987).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2A antigen affinity purified polyclonal antibody (Catalog # A30987) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Rabbit anti-Goat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for AP2A. test
Protein Target Info & Infographic
Gene/Protein Information For AP2A1 (Source: Uniprot.org, NCBI)
Gene Name
AP2A1
Full Name
AP-2 complex subunit alpha-1
Weight
10982 MW
Superfamily
adaptor complexes large subunit family
Alternative Names
100 kDa coated vesicle protein A; Adapter-related protein complex 2 alpha-1 subunit; Adaptor protein complex AP-2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; ADTAAAP2-ALPHA; alpha1-adaptin; Alpha-adaptin A; AP-2 complex subunit alpha-1; CLAPA1adaptin, alpha A; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit AP2A1 ADTAA, AP2-ALPHA, CLAPA1 adaptor related protein complex 2 subunit alpha 1 AP-2 complex subunit alpha-1|100 kDa coated vesicle protein A|adapter-related protein complex 2 alpha-1 subunit|adapter-related protein complex 2 subunit alpha-1|adaptin, alpha A|adaptor protein complex AP-2 subunit alpha-1|adaptor related protein complex 2 alpha 1 subunit|alpha-adaptin A|alpha1-adaptin|clathrin assembly protein complex 2 alpha-A large chain|clathrin-associated/assembly/adaptor protein, large, alpha 1|plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP2A1, check out the AP2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP2A ap2a1-1 Antibody (A30987)
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