Anti-Caspase-7(P11)/CASP7 Antibody

Product Name

Anti-Caspase-7(P11)/CASP7 Antibody

View all Caspase-7 Antibodies

SKU/Catalog Number

PA1442

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-7 (P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-7(P11)/CASP7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1442)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11), different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1442 is reactive to CASP7 in Human, Mouse, Rat

Applications

PA1442 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

34277 MW

Background of Caspase-7

CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Click image to see more details

Anti-Caspase-7(P11) antibody, PA1442, Western blotting
All lanes: Anti CASP7(p11) (PA1442) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 34KD
Observed bind size: 34KD

Click image to see more details

Anti-Caspase-7(P11) antibody, PA1442, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Gene/Protein Information For CASP7 (Source: Uniprot.org, NCBI)

Alternative Names

apoptosis-related cysteine protease; Apoptotic protease Mch-3; CASP7; CASP-7; caspase 7, apoptosis-related cysteine peptidase; Caspase7; Caspase-7; EC 3.4.22; EC 3.4.22.60; ICE-like apoptotic protease 3; Lice2 alpha/beta/gamma; Mch3 CASP7 CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 caspase 7 caspase-7|ICE-like apoptotic protease 3|apoptotic protease MCH-3|caspase 7, apoptosis-related cysteine peptidase|caspase 7, apoptosis-related cysteine protease

For more info on CASP7, check out the CASP7 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CASP7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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