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Key=meta_title, value=Anti-Caspase-7(P11)/CASP7 Antibody
Key=meta_description, value=Boster Bio Anti-Caspase-7(P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Anti-Caspase-7(P11) antibody, PA1442, Western blotting
All lanes: Anti CASP7(p11) (PA1442) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney
Key=small_image_label, value=Anti-Caspase-7(P11) antibody, PA1442, Western blotting
All lanes: Anti CASP7(p11) (PA1442) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney
Key=thumbnail_label, value=Anti-Caspase-7(P11) antibody, PA1442, Western blotting
All lanes: Anti CASP7(p11) (PA1442) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney
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Key=size, value=100 μg/vial
Key=protein_name, value=Caspase-7(CASP-7)
Key=gene_full_name, value=Caspase-7
Key=uniprot_id, value=P55210
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=IHC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Key=predicted_reactivity
Key=source_company, value=Boster PA1442
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=34277 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Apoptosis, Apoptotic Markers, Cancer, Caspases, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes
Key=clonality, value=Polyclonal
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Key=cj_related_products, value=Apoptotic Dna Fragmentation:PB9369|M01044|A01044-2^Execution Phase Of Apoptosis:A01044-2^Release Of Cytochrome C From Mitochondria:A00181
Key=description, value=Boster Bio Anti-Caspase-7 (P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Caspase-7 (P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Caspase-7 (P11)/CASP7 Antibody
Key=contents, value=Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Key=background, value=CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.
Key=gene_name, value=CASP7
Key=synonyms, value=Caspase-7;CASP-7;3.4.22.60;Apoptotic protease Mch-3;CMH-1;ICE-like apoptotic protease 3;ICE-LAP3;Caspase-7 subunit p20;Caspase-7 subunit p11;CASP7;MCH3;
Key=application_details, value=Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Key=subcellular_localization, value= Cytoplasm.
Key=tissue_specificity, value= Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Key=protein_function, value= Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly- 217' bond. Overexpression promotes programmed cell death.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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Key=immunogen, value=A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11), different from the related mouse and rat sequences by two amino acids.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=34 kDa
Key=custom_attribute_2, value=WB: HELA Whole Cell, MCF-7 Whole Cell, Rat Liver Tissue, Rat Kidney Tissue
IHC: Human Intestinal Cancer Tissue
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Anti-Caspase-7(P11)/CASP7 Antibody Picoband®

Caspase-7 antibody

Boster Bio Anti-Caspase-7 (P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 3 publication(s).

Product Info Summary

SKU: PA1442
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Caspase-7(P11)/CASP7 Antibody Picoband®

View all Caspase-7 Antibodies

SKU/Catalog Number

PA1442

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-7 (P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-7(P11)/CASP7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1442)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11), different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1442 is reactive to CASP7 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

34 kDa

Calculated molecular weight

34277 MW

Background of Caspase-7

CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1442 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: HELA Whole Cell, MCF-7 Whole Cell, Rat Liver Tissue, Rat Kidney Tissue
IHC: Human Intestinal Cancer Tissue

Validation Images & Assay Conditions

Gene/Protein Information For CASP7 (Source: Uniprot.org, NCBI)

Gene Name

CASP7

Full Name

Caspase-7

Weight

34277 MW

Superfamily

peptidase C14A family

Alternative Names

Caspase-7;CASP-7;3.4.22.60;Apoptotic protease Mch-3;CMH-1;ICE-like apoptotic protease 3;ICE-LAP3;Caspase-7 subunit p20;Caspase-7 subunit p11;CASP7;MCH3; CASP7 CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 caspase 7 caspase-7|ICE-like apoptotic protease 3|apoptotic protease MCH-3|caspase 7, apoptosis-related cysteine peptidase|caspase 7, apoptosis-related cysteine protease

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CASP7, check out the CASP7 Infographic

CASP7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CASP7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1442 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cartilage-specific deletion of Alk5 gene results in a progressive osteoarthritis-like phenotype in mice

Anemonin attenuates osteoarthritis progression through inhibiting the activation of IL%u20101%u03B2/NF%u2010%u03BAB pathway

Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...

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6 Customer Q&As for Anti-Caspase-7(P11)/CASP7 Antibody Picoband®

Question

Will PA1442 anti-Caspase-7(P11)/CASP7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

As indicated on the product datasheet, PA1442 anti-Caspase-7(P11)/CASP7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-24

Question

I have attached the WB image, lot number and protocol we used for t-cell using anti-Caspase-7(P11)/CASP7 antibody PA1442. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-25

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-7(P11)/CASP7 antibody PA1442. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-12

Question

Is this PA1442 anti-Caspase-7(P11)/CASP7 antibody reactive to the isotypes of CASP7?

Verified Customer

Verified customer

Asked: 2018-04-02

Answer

The immunogen of PA1442 anti-Caspase-7(P11)/CASP7 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11)(237-255aa RGSWFVQALCSILEEHGKD), different from the related mouse and rat sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-04-02

Question

I am interested in to test anti-Caspase-7(P11)/CASP7 antibody PA1442 on rat t-cell for research purposes, then I may be interested in using anti-Caspase-7(P11)/CASP7 antibody PA1442 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Edwards

Verified customer

Asked: 2017-03-21

Answer

The products we sell, including anti-Caspase-7(P11)/CASP7 antibody PA1442, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-03-21

Question

Can you help my question with product PA1442, anti-Caspase-7(P11)/CASP7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Jackson

Verified customer

Asked: 2016-02-01

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1442 anti-Caspase-7(P11)/CASP7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-02-01

Order DetailsPrice
PA1442

100μg

$370
PA1442-10ug

10μg sample (liquid)

$99
PA1442-Biotin

100 μg Biotin conjugated

$570
PA1442-Cy3

100 μg Cy3 conjugated

$570
PA1442-Dylight488

100 μg Dylight488 conjugated

$570
PA1442-Dylight550

100 μg Dylight550 conjugated

$570
PA1442-Dylight594

100 μg Dylight594 conjugated

$570
PA1442-FITC

100 μg FITC conjugated

$570
PA1442-HRP

100 μg HRP conjugated

$570
PA1442-APC

100 μg APC conjugated

$670
PA1442-PE

100 μg PE conjugated

$670
PA1442-iFluor647

100 μg iFluor647 conjugated

$670
PA1442-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1442
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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