Anti-Caspase-7(P11)/CASP7 Antibody Picoband®

Product Name

Anti-Caspase-7(P11)/CASP7 Antibody Picoband®

View all Caspase-7 Antibodies

SKU/Catalog Number

PA1442

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Caspase-7 (P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-7(P11)/CASP7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1442)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11), different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1442 is reactive to CASP7 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

34 kDa

Calculated molecular weight

34277 MW

Background of Caspase-7

CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1442 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: HELA Whole Cell, MCF-7 Whole Cell, Rat Liver Tissue, Rat Kidney Tissue
IHC: Human Intestinal Cancer Tissue

Validation Images & Assay Conditions

Gene/Protein Information For CASP7 (Source: Uniprot.org, NCBI)

Alternative Names

Caspase-7;CASP-7;3.4.22.60;Apoptotic protease Mch-3;CMH-1;ICE-like apoptotic protease 3;ICE-LAP3;Caspase-7 subunit p20;Caspase-7 subunit p11;CASP7;MCH3; CASP7 CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 caspase 7 caspase-7|ICE-like apoptotic protease 3|apoptotic protease MCH-3|caspase 7, apoptosis-related cysteine peptidase|caspase 7, apoptosis-related cysteine protease

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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