Key=store_id Key=entity_id, value=2760 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=PB9369 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2015-04-27 15:09:39 Key=updated_at, value=2024-09-26 00:46:26 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-Caspase-7/CASP7 Antibody Picoband® Key=cj_search_index Key=publication_count, value=4 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1369 Key=yoast_content_score, value=30 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Caspase-7/CASP7 Antibody Key=meta_description, value=Boster Bio Anti-Caspase-7/CASP7 Antibody Picoband® catalog # PB9369. Tested in IHC, WB applications. This antibody reacts with Human, Rat. Supplied as 100μg/vial in Lyophilized form antibody. Key=image, value=/p/b/pb9369-1.jpg Key=small_image, value=/p/b/pb9369-1.jpg Key=thumbnail, value=/p/b/pb9369-1.jpg Key=url_key, value=anti-caspase-7-picoband-trade-antibody-pb9369-boster Key=url_path, value=anti-caspase-7-picoband-trade-antibody-pb9369-boster Key=options_container, value=Product Info Column Key=image_label, value=Anti- Caspase-7 Picoband antibody, PB9369, Western blotting All lanes: Anti Caspase-7(PB9369) at 0.5ug/ml Lane 1: A549 Whole Cell Lysate at 40ug Lane 2: Rat Spleen Tissue Lysate at 50ug Lane 3: Rat Lung Tissue Lysate at 50ug Predicted bind Key=small_image_label, value=Anti- Caspase-7 Picoband antibody, PB9369, Western blotting All lanes: Anti Caspase-7(PB9369) at 0.5ug/ml Lane 1: A549 Whole Cell Lysate at 40ug Lane 2: Rat Spleen Tissue Lysate at 50ug Lane 3: Rat Lung Tissue Lysate at 50ug Predicted bind Key=thumbnail_label, value=Anti- Caspase-7 Picoband antibody, PB9369, Western blotting All lanes: Anti Caspase-7(PB9369) at 0.5ug/ml Lane 1: A549 Whole Cell Lysate at 40ug Lane 2: Rat Spleen Tissue Lysate at 50ug Lane 3: Rat Lung Tissue Lysate at 50ug Predicted bind Key=msrp_display_actual_price_type, value= Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=protein_name, value=Caspase-7 Key=gene_full_name, value=Caspase-7 Key=uniprot_id, value=P55210 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Key=sensitivity Key=applications, value=IHC, WB Key=reactivity, value=Human, Rat Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins. Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). Key=predicted_reactivity Key=source_company, value=Boster PB9369 Key=isotype, value=Rabbit IgG Key=molecular_weight, value=34277 MW Key=sample_size_available, value=30ug for $99, contact us for details Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=Apoptosis, Apoptotic Markers, Cancer, Caspases, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes Key=clonality, value=Polyclonal Key=swatch_image, value=/e/k/ek0770_2.png Key=rating_value, value=100 Key=review_count, value=3 Key=phospho_site Key=mp_exclude_sitemap, value=Yes Key=yoast_focus_keyword, value=CD68 antibody Key=yoast_facebook_image, value=no_selection Key=yoast_twitter_image, value=no_selection Key=cj_related_products, value=Apoptotic Dna Fragmentation:PA1442|A01044-2^Caspases:A02384-1^Execution Phase Of Apoptosis:A01044-2^Ubiquitin Proteasome Pathway:A06639-1 Key=description, value=Boster Bio Anti-Caspase-7/CASP7 Antibody Picoband® catalog # PB9369. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-Caspase-7/CASP7 Antibody Picoband® catalog # PB9369. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-Caspase-7/CASP7 Antibody Picoband® Key=contents, value=Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. Key=background, value=CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway. Key=gene_name, value=CASP7 Key=synonyms, value=Caspase-7;CASP-7;3.4.22.60;Apoptotic protease Mch-3;CMH-1;ICE-like apoptotic protease 3;ICE-LAP3;Caspase-7 subunit p20;Caspase-7 subunit p11;CASP7;MCH3; Key=application_details, value=Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human, Rat
Key=subcellular_localization, value= Cytoplasm. Key=sequence_similarities, value=Belongs to the peptidase C14A family. Key=tissue_specificity, value= Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. Key=protein_function, value= Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly- 217' bond. Overexpression promotes programmed cell death. Key=application_notes, value=WB: The detection limit for Caspase-7 is approximately 0.1ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 133247|sc 133248|sc 135858|sc 22179|sc 271747|sc 28295|sc 33773|sc 365034|sc 56063|sc 56066|sc 56067|sc 56068|sc 6138|sc 73424|sc 81654|sc 81655|sc 81660|sc 8510|sc 8511|sc 8512 Key=immunogen, value=E.coli-derived human CASP7 recombinant protein (Position: A117-D198). Human CASP7 shares 92.7% amino acid (aa) sequence identity with both mouse and rat CASP7. Key=purification, value=Immunogen affinity purified. Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=34 kDa Key=custom_attribute_2, value=WB: A549 Whole Cell, Rat Spleen Tissue, Rat Lung Tissue IHC: Human Mammary Cancer Tissue Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-Caspase-7/CASP7 Antibody Picoband®
Caspase-7 antibody
Boster Bio Anti-Caspase-7/CASP7 Antibody Picoband® catalog # PB9369. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 2 publication(s).
Boster Bio Anti-Caspase-7/CASP7 Antibody Picoband® catalog # PB9369. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-7/CASP7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9369)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CASP7 recombinant protein (Position: A117-D198). Human CASP7 shares 92.7% amino acid (aa) sequence identity with both mouse and rat CASP7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9369 is reactive to CASP7 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
34277 MW
Background of Caspase-7
CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: A549 Whole Cell, Rat Spleen Tissue, Rat Lung Tissue IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Caspase-7 Picoband antibody, PB9369, Western blotting All lanes: Anti Caspase-7(PB9369) at 0.5ug/ml Lane 1: A549 Whole Cell Lysate at 40ug Lane 2: Rat Spleen Tissue Lysate at 50ug Lane 3: Rat Lung Tissue Lysate at 50ug Predicted bind size: 34KD Observed bind size: 34KD
Click image to see more details
Anti-Caspase-7 Picoband antibody, PB9369,IHC(P) IHC(P): Human Mammary Cancer Tissue
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on CASP7, check out the CASP7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anemonin attenuates osteoarthritis progression through inhibiting the activation of IL%u20101%u03B2/NF%u2010%u03BAB pathway
Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...
1 Customer Q&As for Anti-Caspase-7/CASP7 Antibody Picoband®
Question
We are currently using anti-Caspase-7/CASP7 antibody PB9369 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on canine tissues as well?
D. Yang
Verified customer
Asked: 2013-10-30
Answer
The anti-Caspase-7/CASP7 antibody (PB9369) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.