Product Info Summary
SKU: | PB9369 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-7/CASP7 Antibody Picoband®
SKU/Catalog Number
PB9369
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-7/CASP7 Antibody Picoband® catalog # PB9369. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-7/CASP7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9369)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CASP7 recombinant protein (Position: A117-D198). Human CASP7 shares 92.7% amino acid (aa) sequence identity with both mouse and rat CASP7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9369 is reactive to CASP7 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
34277 MW
Background of Caspase-7
CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9369 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: A549 Whole Cell, Rat Spleen Tissue, Rat Lung Tissue
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Caspase-7 Picoband antibody, PB9369, Western blotting
All lanes: Anti Caspase-7(PB9369) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Predicted bind size: 34KD
Observed bind size: 34KD
Click image to see more details
Anti-Caspase-7 Picoband antibody, PB9369,IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CASP7 (Source: Uniprot.org, NCBI)
Gene Name
CASP7
Full Name
Caspase-7
Weight
34277 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-7;CASP-7;3.4.22.60;Apoptotic protease Mch-3;CMH-1;ICE-like apoptotic protease 3;ICE-LAP3;Caspase-7 subunit p20;Caspase-7 subunit p11;CASP7;MCH3; CASP7 CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 caspase 7 caspase-7|ICE-like apoptotic protease 3|apoptotic protease MCH-3|caspase 7, apoptosis-related cysteine peptidase|caspase 7, apoptosis-related cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP7, check out the CASP7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-7/CASP7 Antibody Picoband® (PB9369)
Hello CJ!
PB9369 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anemonin attenuates osteoarthritis progression through inhibiting the activation of IL%u20101%u03B2/NF%u2010%u03BAB pathway
Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...
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Customer Q&As
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1 Customer Q&As for Anti-Caspase-7/CASP7 Antibody Picoband®
Question
We are currently using anti-Caspase-7/CASP7 antibody PB9369 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on canine tissues as well?
D. Yang
Verified customer
Asked: 2013-10-30
Answer
The anti-Caspase-7/CASP7 antibody (PB9369) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-30