Product Info Summary
SKU: | PA1008 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Annexin V/ANXA5 Antibody
SKU/Catalog Number
PA1008
BA0644 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Annexin V/ANXA5 Antibody catalog # PA1008. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Annexin V/ANXA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1008)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Annexin V, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1008 is reactive to ANXA5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
35937 MW
Background of Annexin V
Annexin V also know as endonnexin II (ENX2),or placental protein 4 (PP4). Endonexin II is a member of the family of Ca (2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1008 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Skeletal Muscle Tissue, Rat Ovary Tissue, Rat Lung Tissue, MCF-7 Whole Cell, SMMC Whole Cell, A549 Whole Cell, JURKAT Whole Cell, SGC Whole Cell, HT1080 Whole Cell
IHC: Rat Liver Tissue
ICC: HCT116 Cell, HELA Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Annexin V antibody, PA1008, IHC(P)
IHC(P): Rat Liver Tissue
Click image to see more details
Anti-Annexin V antibody, PA1008, ICC
ICC: HCT116 Cell
Click image to see more details
Anti-Annexin V antibody, PA1008, ICC
ICC: HELA Cell
Click image to see more details
Anti-Annexin V antibody, PA1008, Western blotting
All lanes: Anti Annexin V (PA1008) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SGC Whole Cell Lysate at 40ug
Lane 10: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Protein Target Info & Infographic
Gene/Protein Information For ANXA5 (Source: Uniprot.org, NCBI)
Gene Name
ANXA5
Full Name
Annexin A5
Weight
35937 MW
Superfamily
annexin family
Alternative Names
Annexin A5;Anchorin CII;Annexin V;Annexin-5;Calphobindin I;CBP-I;Endonexin II;Lipocortin V;Placental anticoagulant protein 4;PP4;Placental anticoagulant protein I;PAP-I;Thromboplastin inhibitor;Vascular anticoagulant-alpha;VAC-alpha;ANXA5;ANX5, ENX2, PP4; ANXA5 ANX5, ENX2, HEL-S-7, PP4, RPRGL3 annexin A5 annexin A5|CBP-I|PAP-I|VAC-alpha|anchorin CII|annexin V|annexin-5|calphobindin I|endonexin II|epididymis secretory protein Li 7|lipocortin V|placental anticoagulant protein 4|placental anticoagulant protein I|thromboplastin inhibitor|vascular anticoagulant-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANXA5, check out the ANXA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANXA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Annexin V/ANXA5 Antibody (PA1008)
Hello CJ!
PA1008 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma, alone and/or synergistically
Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells
Xiong Y, Ma Xy, Zhang Z, Shao Zj, Zhang Yy, Zhou Lm. Oncol Lett. 2013 Dec;6(6):1789-1793. Epub 2013 Oct 10. Apoptosis Induced By ??,??-Dimethylacrylshikonin Is Associated With Bcl-2 And Nf-??b In Human Breast Carcinoma Mcf-7 Cells.
Jin H, Liu T, Wang Wx, Xu Jh, Yang Pb, Lu Hx, Sun Qr, Hu Ht. Neurochem Int. 2010 Feb;56(3):417-23. Doi: 10.1016/J.Neuint.2009.11.015. Epub 2009 Nov 24. Protective Effects Of [Gly14]-Humanin On Beta-Amyloid-Induced Pc12 Cell Death By Preventing Mit...
Hu Xj, Dong Ng, Shi Jw, Deng C, Li Hd, Lu Cf. J Biomed Mater Res B Appl Biomater. 2014 Feb;102(2):322-36. Doi: 10.1002/Jbm.B.33010. Epub 2013 Sep 20. Evaluation Of A Novel Tetra-Functional Branched Poly(Ethylene Glycol) Crosslinker For Manufacture...
Ji N, Li J, Wei Z, Kong F, Jin H, Chen X, Li Y, Deng Y. Biomed Res Int. 2015;2015:308475. Doi: 10.1155/2015/308475. Epub 2015 Mar 19. Effect Of Celastrol On Growth Inhibition Of Prostate Cancer Cells Through The Regulation Of Herg Channel In Vitro.
Sun X, Kang H, Yao Y, Chen H, Sun L, An W, Jiang E, Wang S, Hu X. Anticancer Drugs. 2015 Jul;26(6):641-8. Doi: 10.1097/Cad.0000000000000229. Dehydrocostus Lactone Suppressed The Proliferation, Migration, And Invasion Of Colorectal Carcinoma Throug...
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Customer Q&As
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5 Customer Q&As for Anti-Annexin V/ANXA5 Antibody
Question
Can PA1008 be used in immunoprecipitation?
Verified customer
Asked: 2021-01-29
Answer
Unfortunately, the Anti-Annexin V/ANXA5 Antibody (PA1008) hasn't been validated in immunoprecipitation. We suggest to run a pilot test to see if it can work for immunoprecipitation.
Boster Scientific Support
Answered: 2021-01-31
Question
I see that the anti-Annexin V/ANXA5 antibody PA1008 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-05
Question
My question regards to test anti-Annexin V/ANXA5 antibody PA1008 on mouse ovary skin for research purposes, then I may be interested in using anti-Annexin V/ANXA5 antibody PA1008 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Mitchell
Verified customer
Asked: 2019-02-25
Answer
The products we sell, including anti-Annexin V/ANXA5 antibody PA1008, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-25
Question
Would anti-Annexin V/ANXA5 antibody PA1008 work for IHC with ovary skin?
G. Mangal
Verified customer
Asked: 2018-03-01
Answer
According to the expression profile of ovary skin, ANXA5 is highly expressed in ovary skin. So, it is likely that anti-Annexin V/ANXA5 antibody PA1008 will work for IHC with ovary skin.
Boster Scientific Support
Answered: 2018-03-01
Question
I have a question about product PA1008, anti-Annexin V/ANXA5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Moore
Verified customer
Asked: 2016-09-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1008 anti-Annexin V/ANXA5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-09-01