Anti-Annexin V/ANXA5 Antibody Picoband®

Annexin V antibody

Boster Bio Anti-Annexin V/ANXA5 Antibody Picoband® catalog # PB9044. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 9 publication(s).

Product Info Summary

SKU: PB9044
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-Annexin V/ANXA5 Antibody Picoband®

View all Annexin V Antibodies

SKU/Catalog Number

PB9044

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Annexin V/ANXA5 Antibody Picoband® catalog # PB9044. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Annexin V/ANXA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9044)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9044 is reactive to ANXA5 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

36 kDa

Calculated molecular weight

35937 MW

Background of Annexin V

Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9044 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat, -
Immunofluorescence, 2μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: Rat Lung Tissue, Rat Brain Tissue, Rat Cardiac Muscle Tissue, Rat Kidney Tissue, HELA Whole Cell, SMMC Whole Cell, A549 Whole Cell, SGC Whole Cell,
IHC: Human Intestinal Cancer tissue, Human Mammary Cancer tissue, Mouse Kidney tissue, Rat Kidney tissue
IHC-F: rat kidney tissue, human placenta tissue
ICC/IF: U20S cell, RH35 cell
IF: human placenta cancer tissue
FCM: PC-3 cell, SiHa cell

Validation Images & Assay Conditions

Gene/Protein Information For ANXA5 (Source: Uniprot.org, NCBI)

Gene Name

ANXA5

Full Name

Annexin A5

Weight

35937 MW

Superfamily

annexin family

Alternative Names

Annexin A5;Anchorin CII;Annexin V;Annexin-5;Calphobindin I;CBP-I;Endonexin II;Lipocortin V;Placental anticoagulant protein 4;PP4;Placental anticoagulant protein I;PAP-I;Thromboplastin inhibitor;Vascular anticoagulant-alpha;VAC-alpha;ANXA5;ANX5, ENX2, PP4; ANXA5 ANX5, ENX2, HEL-S-7, PP4, RPRGL3 annexin A5 annexin A5|CBP-I|PAP-I|VAC-alpha|anchorin CII|annexin V|annexin-5|calphobindin I|endonexin II|epididymis secretory protein Li 7|lipocortin V|placental anticoagulant protein 4|placental anticoagulant protein I|thromboplastin inhibitor|vascular anticoagulant-alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ANXA5, check out the ANXA5 Infographic

ANXA5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ANXA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9044 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma,alone and/or synergistically

Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells

Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma, alone and/or synergistically

Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells

Xiong Y, Ma Xy, Zhang Z, Shao Zj, Zhang Yy, Zhou Lm. Oncol Lett. 2013 Dec;6(6):1789-1793. Epub 2013 Oct 10. Apoptosis Induced By ??,??-Dimethylacrylshikonin Is Associated With Bcl-2 And Nf-??b In Human Breast Carcinoma Mcf-7 Cells.

Jin H, Liu T, Wang Wx, Xu Jh, Yang Pb, Lu Hx, Sun Qr, Hu Ht. Neurochem Int. 2010 Feb;56(3):417-23. Doi: 10.1016/J.Neuint.2009.11.015. Epub 2009 Nov 24. Protective Effects Of [Gly14]-Humanin On Beta-Amyloid-Induced Pc12 Cell Death By Preventing Mit...

Hu Xj, Dong Ng, Shi Jw, Deng C, Li Hd, Lu Cf. J Biomed Mater Res B Appl Biomater. 2014 Feb;102(2):322-36. Doi: 10.1002/Jbm.B.33010. Epub 2013 Sep 20. Evaluation Of A Novel Tetra-Functional Branched Poly(Ethylene Glycol) Crosslinker For Manufacture...

Ji N, Li J, Wei Z, Kong F, Jin H, Chen X, Li Y, Deng Y. Biomed Res Int. 2015;2015:308475. Doi: 10.1155/2015/308475. Epub 2015 Mar 19. Effect Of Celastrol On Growth Inhibition Of Prostate Cancer Cells Through The Regulation Of Herg Channel In Vitro.

Sun X, Kang H, Yao Y, Chen H, Sun L, An W, Jiang E, Wang S, Hu X. Anticancer Drugs. 2015 Jul;26(6):641-8. Doi: 10.1097/Cad.0000000000000229. Dehydrocostus Lactone Suppressed The Proliferation, Migration, And Invasion Of Colorectal Carcinoma Throug...

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1 Customer Q&As for Anti-Annexin V/ANXA5 Antibody Picoband®

Question

We are currently using anti-Annexin V/ANXA5 antibody PB9044 for rat tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-05

Answer

The anti-Annexin V/ANXA5 antibody (PB9044) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-05

Order DetailsPrice
PB9044

100μg

$370
PB9044-10ug

10μg sample (liquid)

$99
PB9044-Biotin

100 μg Biotin conjugated

$570
PB9044-Cy3

100 μg Cy3 conjugated

$570
PB9044-Dylight488

100 μg Dylight488 conjugated

$570
PB9044-Dylight550

100 μg Dylight550 conjugated

$570
PB9044-Dylight594

100 μg Dylight594 conjugated

$570
PB9044-FITC

100 μg FITC conjugated

$570
PB9044-HRP

100 μg HRP conjugated

$570
PB9044-APC

100 μg APC conjugated

$670
PB9044-PE

100 μg PE conjugated

$670
PB9044-iFluor647

100 μg iFluor647 conjugated

$670
PB9044-carrier-free

Carrier Free

$370

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