Product Info Summary
SKU: | RP1037 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-Angiopoietin 2/ANGPT2 Antibody
View all Angiopoietin-2 Antibodies
SKU/Catalog Number
RP1037
PB0093 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiopoietin 2/ANGPT2 Antibody catalog # RP1037. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiopoietin 2/ANGPT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1037)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Angiopoietin 2 recombinant protein (Position: Y19-N348). Human Angiopoietin 2 shares 84% and 85% amino acid (aa) sequences identity with mouse and rat Angiopoietin 2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1037 is reactive to ANGPT2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
125 kDa
Calculated molecular weight
56919 MW
Background of Angiopoietin-2
ANGPT2, also known as ANG2 or Angiopoietin 2, is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema, tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1037 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
IHC: Human Placenta Tissue, Rat Intestine Tissue, Mouse Intestine Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Angiopoietin 2 Picoband antibody, RP1037-1.JPG
IHC(P): Human Placenta Tissue
Click image to see more details
Anti-Angiopoietin 2 Picoband antibody, RP1037-2.JPG
IHC(P): Rat Intestine Tissue
Click image to see more details
Anti-Angiopoietin 2 Picoband antibody, RP1037-3.JPG
IHC(P): Mouse Intestine Tissue
Protein Target Info & Infographic
Gene/Protein Information For ANGPT2 (Source: Uniprot.org, NCBI)
Gene Name
ANGPT2
Full Name
Angiopoietin-2
Weight
56919 MW
Alternative Names
Angiopoietin-2;ANG-2;ANGPT2; ANGPT2 AGPT2, ANG2 angiopoietin 2 angiopoietin-2|Tie2-ligand|angiopoietin-2B|angiopoietin-2a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANGPT2, check out the ANGPT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANGPT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiopoietin 2/ANGPT2 Antibody (RP1037)
Hello CJ!
RP1037 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Benefical Therapeutic Effect of Chinese Herbal Xinji'erkang Formula on Hypertension-Induced Renal Injury in the 2-Kidney-1-Clip Hypertensive Rats
Li C, Fan J, Song X, Zhang B, Chen Y, Li C, Mi K, Ma H, Song Y, Tao X, Li G. Plos One. 2013 Sep 11;8(9):E75388. Doi: 10.1371/Journal.Pone.0075388. Ecollection 2013. Expression Of Angiopoietin-2 And Vascular Endothelial Growth Factor Receptor-3 Cor...
Bai X, Li X, Tian J, Zhou Z. Plos One. 2014 Apr 23;9(4):E96117. Doi: 10.1371/Journal.Pone.0096117. Ecollection 2014. Antiangiogenic Treatment Diminishes Renal Injury And Dysfunction Via Regulation Of Local Akt In Early Experimental Diabetes.
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Customer Q&As
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1 Customer Q&As for Anti-Angiopoietin 2/ANGPT2 Antibody
Question
We are currently using anti-Angiopoietin 2/ANGPT2 antibody RP1037 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
K. Evans
Verified customer
Asked: 2013-02-27
Answer
The anti-Angiopoietin 2/ANGPT2 antibody (RP1037) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-27