Click image to see more details
Product Info Summary
| SKU: | EK0938 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Angiopoietin-2 ELISA Kit PicoKine®
View all Angiopoietin-2 ELISA kits
SKU/Catalog Number
EK0938
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Angiopoietin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Angpt2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Angiopoietin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0938)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: M1-F496
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0938 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0938 is reactive to Angpt2 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0938 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angiopoietin-2
Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0938-CAP | Anti-Mouse Angpt2 Pre-coated 96-well strip microplate | 1 |
| EK0938-ST | Mouse Angpt2 Standard | 2 vials, 10 ng/tube |
| EK0938-DA | Mouse Angpt2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.028 | 0.102 | 0.152 | 0.264 | 0.529 | 0.855 | 1.234 | 1.916 |
Data Example Images
Click image to see more details
Mouse Angiopoietin-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Angiopoietin-2, Dilution ratio of 1:10, concentration in serum and plasma is 10-20 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Angiopoietin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 210 | 1256 | 4765 | 230 | 1185 | 4889 |
| Standard deviation | 10.5 | 55.06 | 274.67 | 11.50 | 54.51 | 303.11 |
| CV (%) | 5% | 4.4% | 5.8% | 5% | 4.6% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 210 | 228 | 239 | 234 | 227 | 10.96 | 4.8% |
| Sample 2 | 1256 | 1343 | 1241 | 1350 | 1297 | 49.34 | 3.8% |
| Sample 3 | 4765 | 5564 | 5203 | 5667 | 5299 | 353.56 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For Angpt2 (Source: Uniprot.Org, NCBI)
Gene Name
Angpt2
Full Name
Angiopoietin-2
Weight
56576 MW
Alternative Names
Angiopoietin-2;ANG-2;Angpt2;Agpt2; ANGPT2 AGPT2, ANG2 angiopoietin 2 angiopoietin-2|Tie2-ligand|angiopoietin-2B|angiopoietin-2a
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Angpt2, check out the Angpt2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Angpt2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Angiopoietin-2 ELISA Kit PicoKine® (EK0938)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse Angiopoietin-2 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Mouse Angiopoietin-2 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
12 Customer Q&As for Mouse Angiopoietin-2 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-10-28
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-10-28
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Zhang
Verified customer
Asked: 2020-04-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-17
Question
Q: if the enzyme conjugated Angiopoietin-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Angiopoietin-2 antigen?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-09-19
Question
Q: What is the optimal O.D. value for Angiopoietin-2 ELISA kit? I used your Angiopoietin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Angiopoietin-2 even though the O.D. values are not very high?
R. Green
Verified customer
Asked: 2019-09-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Angiopoietin-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Angiopoietin-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-09-03
Question
Has this kit been tested in rat or the homology of the antibody immunogens with rat?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
No. This kit has neither been tested in rat nor the homology of the antibody immunogens with rat. Since the mouse ANG-2 and rat ANG-2 are 100% the same, the mouse ANG-2 ELISA kit should work for rat ANG-2.
Boster Scientific Support
Answered: 2019-06-13
Question
Can this kit be used for the sheep samples? What is the amino acid homologies between the immunogen sequences of capture- and detection-antibodies and sheep protein?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
It is unlikely the kit can be used to detect the sheep samples. The homology is about 80%.
Boster Scientific Support
Answered: 2019-06-10
Question
Q: can you recommend the dilution ratio of serum samples for detection of Angiopoietin-2 in Mouse cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
K. Garcia
Verified customer
Asked: 2018-11-25
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Angiopoietin-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-25
Question
Q: which procedure should I follow in order to thaw whole blood sample for Angiopoietin-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Angiopoietin-2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-03-20
Question
Q: how do I analyze ELISA data? I measured Angiopoietin-2 level in cell lysates.
M. Johnson
Verified customer
Asked: 2017-04-16
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-04-16
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2017-03-03
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-03-03
Question
Q: Can Angiopoietin-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2016-07-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-07-07
Question
My Angiopoietin-2 levels on normal mouse serum detected by the kit are very high. Does this kit detect Angiopoietin-1 or any other homologs? For this kit used on the normal mouse serum, what is the normal Angiopoietin-2 detection range?
Verified Customer
Verified customer
Asked: 2014-05-26
Answer
This kit only detects Angiopoietin-2 and has shown no cross reactivity with Angiopoietin 1 or Tie 2 (soluble Tie 2 receptor). Our Angiopoietin-2 detection range is around 20-50 ng/mL, and therefore we consider your detection values were in the normal range. Although the written highest point is 10 ng/mL, this kit can detect even higher. We suggest you perform a dilution gradient to determine the appropriate detection range.
Boster Scientific Support
Answered: 2014-05-26
