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Product Info Summary
| SKU: | A01818-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Mouse CD90/Thy1 DyLight® 550 conjugated Antibody
SKU/Catalog Number
A01818-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Mouse CD90/Thy1 DyLight® 550 conjugated Antibody catalog # A01818-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Mouse.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Mouse CD90/Thy1 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01818-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01818-Dyl550 is reactive to Thy1 in Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
17.935kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01818-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For Thy1 (Source: Uniprot.org, NCBI)
Gene Name
Thy1
Full Name
Thy-1 membrane glycoprotein
Weight
17.935kDa
Alternative Names
Thy-1 membrane glycoprotein; Thy-1 antigen; CD90; Thy1; Thy-1 THY1 CD90, CDw90 Thy-1 cell surface thy-1 membrane glycoprotein|Thy-1 T-cell |thy-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Thy1, check out the Thy1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Thy1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mouse CD90/Thy1 DyLight® 550 conjugated Antibody (A01818-Dyl550)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Mouse CD90/Thy1 DyLight® 550 conjugated Antibody
Question
We have observed staining in mouse eye. Are there any suggestions? Is anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody supposed to stain eye positively?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
From literature eye does express THY1. From Uniprot.org, THY1 is expressed in anterior cingulate cortex, brain, amygdala, eye, liver, leukemic t-cell, among other tissues. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2020-03-23
Question
We are currently using anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550 for mouse tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody (A01818-Dyl550) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-22
Question
Will anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550 work for Flow Cytometry with amygdala?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
According to the expression profile of amygdala, THY1 is highly expressed in amygdala. So, it is likely that anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550 will work for Flow Cytometry with amygdala.
Boster Scientific Support
Answered: 2020-01-06
Question
Is this A01818-Dyl550 anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody reactive to the isotypes of THY1?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The immunogen of A01818-Dyl550 anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody is E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-14
Question
I see that the anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-06
Question
I was wanting to use your anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody for Flow Cytometry for mouse amygdala on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse amygdala identification?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
You can see on the product datasheet, A01818-Dyl550 anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody has been tested for Flow Cytometry on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse amygdala in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-11
Question
Does A01818-Dyl550 anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
It shows on the product datasheet, A01818-Dyl550 anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-17
Question
Our team were happy with the WB result of your anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody. However we have been able to see positive staining in eye cell membrane using this antibody. Is that expected? Could you tell me where is THY1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-05
Answer
From what I have seen in literature, eye does express THY1. Generally THY1 expresses in cell membrane. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2018-09-05
Question
I have a question about product A01818-Dyl550, anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01818-Dyl550 anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-22
Question
My lab would like to test anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550 on mouse amygdala for research purposes, then I may be interested in using anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
The products we sell, including anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-05
Question
Here is the WB image, lot number and protocol we used for amygdala using anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550. Please let me know if you require anything else.
O. Rodriguez
Verified customer
Asked: 2016-01-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-18
Question
Is a blocking peptide available for product anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody (A01818-Dyl550)?
B. Taylor
Verified customer
Asked: 2015-10-22
Answer
We do provide the blocking peptide for product anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody (A01818-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-10-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550. Let me know if you need anything else.
A. Parker
Verified customer
Asked: 2015-04-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-08
Question
Is there a BSA free version of anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550 available?
P. Li
Verified customer
Asked: 2013-07-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Mouse CD90/Thy1 DyLight® 550 conjugated antibody A01818-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-07-01
