Product Info Summary
SKU: | A03756-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-alpha Internexin/INA Antibody Picoband™
View all alpha-Internexin Antibodies
SKU/Catalog Number
A03756-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-alpha Internexin/INA Antibody Picoband™ catalog # A03756-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha Internexin/INA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03756-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human INA recombinant protein (Position: L62-D169).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03756-1 is reactive to INA in Human, Mouse, Rat
Applications
A03756-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
66 kDa
Calculated molecular weight
Background of alpha-Internexin
Alpha-Internexin (INA; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Immunofluorescence, 5μg/ml, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
![a03756 1 ina primary antibodies wb testing 1 a03756 1 ina primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03756-1-ina-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Internexin/INA antigen affinity purified polyclonal antibody (Catalog # A03756-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Internexin/INA at approximately 66 kDa. The expected band size for Alpha Internexin/INA is at 66 kDa.
![a03756 1 ina primary antibodies ihc testing 2 a03756 1 ina primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a03756-1-ina-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1).
Alpha Internexin/INA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Internexin/INA Antibody (A03756-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a03756 1 ina primary antibodies ihc testing 3 a03756 1 ina primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a03756-1-ina-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1).
Alpha Internexin/INA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Internexin/INA Antibody (A03756-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a03756 1 ina primary antibodies ihc testing 4 a03756 1 ina primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a03756-1-ina-primary-antibodies-ihc-testing-4.jpg)
Click image to see more details
Figure 4. IHC analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1).
Alpha Internexin/INA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Internexin/INA Antibody (A03756-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a03756 1 ina primary antibodies if testing 5 a03756 1 ina primary antibodies if testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a03756-1-ina-primary-antibodies-if-testing-5.jpg)
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Figure 5. IF analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1).
Alpha Internexin/INA was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Alpha Internexin/INA Antibody (A03756-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a03756 1 ina primary antibodies if testing 6 a03756 1 ina primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a03756-1-ina-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1).
Alpha Internexin/IN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Alpha Internexin/INA Antibody (A03756-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a03756 1 ina primary antibodies fcm testing 7 a03756 1 ina primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a03756-1-ina-primary-antibodies-fcm-testing-7.png)
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Figure 7. Flow Cytometry analysis of A549 cells using anti-Alpha Internexin/INA antibody (A03756-1).
Overlay histogram showing A549 cells stained with A03756-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Internexin/INA Antibody (A03756-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For INA (Source: Uniprot.org, NCBI)
Gene Name
INA
Full Name
Alpha-internexin
Weight
Superfamily
intermediate filament family
Alternative Names
66 kDa neurofilament protein; alphaInternexin; alpha-Internexin; alpha-Inx; FLJ18662; FLJ57501; INA; internexin neuronal intermediate filament protein, alpha; MGC12702; NEF5; neurofilament 5 (66kD); Neurofilament 5; Neurofilament-66; neurofilament-66, tax-binding protein; NF-66; NF-66alpha-internexin; TXBP-1 INA NEF5, NF-66, TXBP-1 internexin neuronal intermediate filament protein alpha alpha-internexin|66 kDa neurofilament protein|alpha-Inx|neurofilament 5 (66kD)|neurofilament-66, tax-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INA, check out the INA Infographic
![INA infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha Internexin/INA Antibody Picoband™ (A03756-1)
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