Click image to see more details
Product Info Summary
| SKU: | PB9589 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FMO1 Antibody Picoband®
SKU/Catalog Number
PB9589
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FMO1 Antibody Picoband® catalog # PB9589. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FMO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9589)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FMO1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9589 is reactive to FMO1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
60311 MW
Background of FMO1
Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9589 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Liver Tissue, Mouse Liver Tissue, Rat Kidney Tissue, Mouse Kidney Tissue, SMMC Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FMO1 using anti-FMO1 antibody (PB9589).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: Mouse Liver Tissue Lysate at 50ug,
Lane 3: Rat Kidney Tissue Lysate at 50ug,
Lane 4: Mouse Kidney Tissue Lysate at 50ug,
Lane 5: SMMC Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO1 antigen affinity purified polyclonal antibody (Catalog # PB9589) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO1 at approximately 60 kDa. The expected band size for FMO1 is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FMO1 (Source: Uniprot.org, NCBI)
Gene Name
FMO1
Full Name
Dimethylaniline monooxygenase [N-oxide-forming] 1
Weight
60311 MW
Superfamily
FMO family
Alternative Names
Dimethylaniline monooxygenase [N-oxide-forming] 1;1.14.13.8;Dimethylaniline oxidase 1;Fetal hepatic flavin-containing monooxygenase 1;FMO 1;FMO1; FMO1 flavin containing dimethylaniline monoxygenase 1 dimethylaniline monooxygenase [N-oxide-forming] 1|FMO 1|Flavin-containing monooxygenase 1 (fetal liver)|dimethylaniline oxidase 1|fetal hepatic flavin-containing monooxygenase 1|flavin containing monooxygenase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FMO1, check out the FMO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FMO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FMO1 Antibody Picoband® (PB9589)
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Customer Q&As
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6 Customer Q&As for Anti-FMO1 Antibody Picoband®
Question
Does anti-FMO1 antibody PB9589 work on horse WB with nephron tubule?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Our lab technicians have not validated anti-FMO1 antibody PB9589 on horse. You can run a BLAST between horse and the immunogen sequence of anti-FMO1 antibody PB9589 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse nephron tubule in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-06
Question
I see that the anti-FMO1 antibody PB9589 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-16
Question
See attached the WB image, lot number and protocol we used for thalamus using anti-FMO1 antibody PB9589. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-02
Question
Can you help my question with product PB9589, anti-FMO1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9589 anti-FMO1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-15
Question
Does PB9589 anti-FMO1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Jackson
Verified customer
Asked: 2014-10-29
Answer
It shows on the product datasheet, PB9589 anti-FMO1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-10-29
Question
We are currently using anti-FMO1 antibody PB9589 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
J. Huang
Verified customer
Asked: 2014-06-23
Answer
The anti-FMO1 antibody (PB9589) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-23
