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Product Info Summary
| SKU: | A04248-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband®
View all Serpin F2/alpha 2-Antiplasmin Antibodies
SKU/Catalog Number
A04248-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® catalog # A04248-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04248-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Alpha 2 Antiplasmin/SERPINF2 recombinant protein (Position: K461-K491).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04248-2 is reactive to SERPINF2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
54.566kDa
Background of Serpin F2/alpha 2-Antiplasmin
Alpha 2-antiplasmin (or α2-antiplasmin or plasmin inhibitor) is a serine protease inhibitor (serpin) responsible for inactivating plasmin. Plasmin is an important enzyme that participates in fibrinolysis and degradation of various other proteins. This protein is encoded by the SERPINF2 gene. This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04248-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human HepG2 whole cell, human K562 whole cell, human HCCT tissue
IHC: human liver cancer tissue, human lymphoma tissue, human stomach cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HCCT tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 2 Antiplasmin/SERPINF2 antigen affinity purified polyclonal antibody (Catalog # A04248-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 2 Antiplasmin/SERPINF2 at approximately 65 kDa. The expected band size for Alpha 2 Antiplasmin/SERPINF2 is at 65 kDa.
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Figure 2. IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2).
Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2).
Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2).
Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SERPINF2 (Source: Uniprot.org, NCBI)
Gene Name
SERPINF2
Full Name
Alpha-2-antiplasmin
Weight
54.566kDa
Superfamily
serpin family
Alternative Names
Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD23; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; FCER2; CD23A; CLEC4J; FCE2; IGEBF SERPINF2 A2AP, AAP, ALPHA-2-PI, API, PLI, alpha2AP serpin family F member 2 alpha-2-antiplasmin|alpha-2-AP|alpha-2-plasmin inhibitor|plasmin inhibitor|serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2|serpin F2|serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINF2, check out the SERPINF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® (A04248-2)
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