Click image to see more details
Product Info Summary
| SKU: | A03438-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Ager Antibody Picoband®
SKU/Catalog Number
A03438-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ager Antibody Picoband® catalog # A03438-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Ager Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03438-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Ager recombinant protein (Position: Q24-E320).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03438-2 is reactive to Ager in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
43 kDa, 58 kDa
Calculated molecular weight
31203 MW
Background of AGER
Enables S100 protein binding activity; advanced glycation end-product binding activity; and heparin binding activity. Involved in several processes, including cellular response to amyloid-beta; negative regulation of long-term synaptic potentiation; and positive regulation of cytokine production. Acts upstream of or within several processes, including induction of positive chemotaxis; negative regulation of advanced glycation end-product receptor activity; and positive regulation of macromolecule metabolic process. Located in extracellular space and plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and lung. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); cardiovascular system disease (multiple); cystic fibrosis; kidney failure (multiple); and lupus nephritis. Orthologous to human AGER (advanced glycosylation end-product specific receptor).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03438-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat lung tissue, mouse lung tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Ager using anti-Ager antibody (A03438-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ager antigen affinity purified polyclonal antibody (Catalog # A03438-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ager at approximately 43,58 kDa. The expected band size for Ager is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Ager (Source: Uniprot.org, NCBI)
Gene Name
Ager
Full Name
Advanced glycosylation end product-specific receptor
Weight
31203 MW
Alternative Names
Tumor necrosis factor-inducible gene 6 protein;Hyaluronate-binding protein;TNF-stimulated gene 6 protein;TSG-6;Tumor necrosis factor alpha-induced protein 6;TNF alpha-induced protein 6;TNFAIP6;TSG6; AGER RAGE, SCARJ1, sRAGE advanced glycosylation end-product specific receptor advanced glycosylation end product-specific receptor|receptor for advanced glycation end-products variant 20
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ager, check out the Ager Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ager: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ager Antibody Picoband® (A03438-2)
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