Product Info Summary
SKU: | EK0828 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse RAGE ELISA Kit PicoKine®
SKU/Catalog Number
EK0828
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse RAGE ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ager in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse RAGE ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0828)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q24-A342
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0828 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0828 is reactive to Ager in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0828 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of AGER
RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK0828-CAP | Anti-Mouse Ager Pre-coated 96-well strip microplate | 1 |
EK0828-ST | Mouse Ager Standard | 2 vials, 10 ng/tube |
EK0828-DA | Mouse Ager Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.064 | 0.169 | 0.232 | 0.339 | 0.567 | 0.965 | 1.465 | 1.947 |
Data Example Images
Click image to see more details
Mouse RAGE PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect AGER, Dilution ratio of 1:5, concentration in serum is 764-1202 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse RAGE ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 113 | 797 | 2158 | 103 | 830 | 2180 |
Standard deviation | 4.63 | 55.79 | 90.63 | 6%5 | 61.42 | 130.8 |
CV (%) | 4.1% | 7% | 4.2% | 5% | 7.4% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of AGER in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 113 | 119 | 116 | 113 | 115 | 2.48 | 2.1% |
Sample 2 | 797 | 796 | 771 | 805 | 792 | 12.75 | 1.6% |
Sample 3 | 2158 | 2179 | 2112 | 2263 | 2178 | 54.73 | 2.5% |
Protein Target Info & Infographic
Gene/Protein Information For Ager (Source: Uniprot.Org, NCBI)
Gene Name
Ager
Full Name
Advanced glycosylation end product-specific receptor
Weight
42669 MW
Alternative Names
Advanced glycosylation end product-specific receptor;Receptor for advanced glycosylation end products;Ager;Rage; AGER RAGE, SCARJ1, sRAGE advanced glycosylation end-product specific receptor advanced glycosylation end product-specific receptor|receptor for advanced glycation end-products variant 20
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ager, check out the Ager Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ager: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse RAGE ELISA Kit PicoKine® (EK0828)
Hello CJ!
EK0828 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu L, Zang P, Feng B, Qian Q. Diabetol Metab Syndr. 2014 Sep 23;6(1):102. Doi: 10.1186/1758-5996-6-102. Ecollection 2014. Atorvastatin Inhibits The Expression Of Rage Induced By Advanced Glycation End Products On Aortas In Healthy Sprague-Dawley R...
Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...
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Customer Q&As
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7 Customer Q&As for Mouse RAGE ELISA Kit PicoKine®
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
C. Kulkarni
Verified customer
Asked: 2020-08-31
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-08-31
Question
Q: which procedure should I follow in order to thaw whole blood sample for AGER ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test AGER for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-03-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Jones
Verified customer
Asked: 2019-12-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-12-16
Question
Q: What is the optimal O.D. value for AGER ELISA kit? I performed your AGER ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain AGER even though the O.D. values are not very high?
W. Lewis
Verified customer
Asked: 2019-04-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this AGER ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of AGER that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-04-30
Question
Q: if the enzyme conjugated AGER antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the AGER antigen?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-06
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
L. Zhao
Verified customer
Asked: 2018-04-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis AGER using the EK0828 Mouse AGER Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-05-05
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2016-05-05