Click image to see more details
Product Info Summary
| SKU: | A04771 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AGAP2 Antibody
SKU/Catalog Number
A04771
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-AGAP2 Antibody (Catalog # A04771). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-AGAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04771)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04771 is reactive to AGAP2 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
37564 MW
Background of Agap2
AGAP2 (also known as Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. Anti-AGAP2 Antibody is useful for researchers interested in Developmental Biology, apoptosis, Cancer, Immunology, and Nuclear Signaling research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04771 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:40,000 - 1:200,000
WB: 1:5,000 - 1:20,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AGAP2 using anti-AGAP2 antibody (A04771).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGAP2 antigen affinity purified polyclonal antibody (Catalog # A04771) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for AGAP2.
Protein Target Info & Infographic
Gene/Protein Information For AGAP2 (Source: Uniprot.org, NCBI)
Gene Name
AGAP2
Full Name
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Weight
37564 MW
Superfamily
centaurin gamma-like family
Alternative Names
AGAP2, AGAP-2, AGAP 2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2, centaurin gamma1, Centg1 Agap2|Centg1, Pike|ArfGAP with GTPase domain, ankyrin repeat and PH domain 2|arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2|AGAP-2|centaurin, gamma 1|cnt-g1|nuclear GTPase PIKE|phosphatidylinositol 3-kinase enhancer
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGAP2, check out the AGAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AGAP2 Antibody (A04771)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AGAP2 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-AGAP2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-AGAP2 Antibody
Question
Will anti-AGAP2 antibody A04771 work for WB with heart?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
According to the expression profile of heart, AGAP2 is highly expressed in heart. So, it is likely that anti-AGAP2 antibody A04771 will work for WB with heart.
Boster Scientific Support
Answered: 2020-04-28
Question
I have a question about product A04771, anti-AGAP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04771 anti-AGAP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-12
Question
Would anti-AGAP2 antibody A04771 work on monkey WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
Our lab technicians have not validated anti-AGAP2 antibody A04771 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-AGAP2 antibody A04771 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-23
Question
We are currently using anti-AGAP2 antibody A04771 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The anti-AGAP2 antibody (A04771) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-14
Question
Would A04771 anti-AGAP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
You can see on the product datasheet, A04771 anti-AGAP2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-15
Question
Is a blocking peptide available for product anti-AGAP2 antibody (A04771)?
T. Wu
Verified customer
Asked: 2019-01-29
Answer
We do provide the blocking peptide for product anti-AGAP2 antibody (A04771). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-AGAP2 antibody A04771. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-16
Question
Is this A04771 anti-AGAP2 antibody reactive to the isotypes of AGAP2?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
The immunogen of A04771 anti-AGAP2 antibody is This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-13
Question
I was wanting to use your anti-AGAP2 antibody for WB for mouse heart on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse heart identification?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
You can see on the product datasheet, A04771 anti-AGAP2 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-26
Question
I see that the anti-AGAP2 antibody A04771 works with WB, what is the protocol used to produce the result images on the product page?
B. Thomas
Verified customer
Asked: 2016-11-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-11-24
Question
Is there a BSA free version of anti-AGAP2 antibody A04771 available?
T. Huang
Verified customer
Asked: 2016-09-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-AGAP2 antibody A04771 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-09-20
Question
Here is the WB image, lot number and protocol we used for heart using anti-AGAP2 antibody A04771. Please let me know if you require anything else.
S. Kulkarni
Verified customer
Asked: 2016-05-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-20
Question
Our lab want to know about to test anti-AGAP2 antibody A04771 on mouse heart for research purposes, then I may be interested in using anti-AGAP2 antibody A04771 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Li
Verified customer
Asked: 2015-07-22
Answer
The products we sell, including anti-AGAP2 antibody A04771, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-07-22
