Product Info Summary
SKU: | M00869 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ADAR1 Monoclonal Antibody
SKU/Catalog Number
M00869
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ADAR1 Monoclonal Antibody catalog # M00869. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00869)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFCB-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ADAR1 Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00869 is reactive to ADAR in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
150 kDa
Calculated molecular weight
38604 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00869 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human Raji whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ADAR using anti-ADAR antibody (M00869).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAR antigen affinity purified monoclonal antibody (Catalog # M00869) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAR at approximately 150 kDa. The expected band size for ADAR is at 150 kDa.
Click image to see more details
Figure 2. IF analysis of ADAR using anti-ADAR antibody (M00869) and anti-Beta Tubulin antibody (M01857-3).
ADAR was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:100 with rabbit anti-ADAR Antibody (M00869) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ADAR (Source: Uniprot.org, NCBI)
Gene Name
ADAR
Full Name
Double-stranded RNA-specific adenosine deaminase
Weight
38604 MW
Alternative Names
Annexin A2;Annexin II;Annexin-2;Calpactin I heavy chain;Calpactin-1 heavy chain;Chromobindin-8;Lipocortin II;Placental anticoagulant protein IV;PAP-IV;Protein I;p36;ANXA2;ANX2, ANX2L4, CAL1H, LPC2D; ADAR ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136 adenosine deaminase RNA specific double-stranded RNA-specific adenosine deaminase|136 kDa double-stranded RNA-binding protein|adenosine deaminase acting on RNA 1-A|dsRNA adenosine deaminase|dsRNA adeonosine deaminase|interferon-induced protein 4|interferon-inducible protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAR, check out the ADAR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAR1 Monoclonal Antibody (M00869)
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No publications found for M00869
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Customer Q&As
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4 Customer Q&As for Anti-ADAR1 Monoclonal Antibody
Question
Is a blocking peptide available for product anti-ADAR1 Monoclonal antibody (M00869)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
We do provide the blocking peptide for product anti-ADAR1 Monoclonal antibody (M00869). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-01
Question
Would M00869 anti-ADAR1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
It shows on the product datasheet, M00869 anti-ADAR1 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-29
Question
Can you help my question with product M00869, anti-ADAR1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Wu
Verified customer
Asked: 2017-09-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00869 anti-ADAR1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-05
Question
Would anti-ADAR1 Monoclonal antibody M00869 work on zebrafish WB with lymph?
B. Rodriguez
Verified customer
Asked: 2015-09-10
Answer
Our lab technicians have not validated anti-ADAR1 Monoclonal antibody M00869 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ADAR1 Monoclonal antibody M00869 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lymph in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-10