Product Info Summary
SKU: | A01810 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ADARB1/Red1 Antibody
SKU/Catalog Number
A01810
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ADARB1/Red1 Antibody (Catalog # A01810). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADARB1/Red1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01810)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ADARB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01810 is reactive to ADARB1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
80763 MW
Background of RED1
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
Mittaz L., Genomics 41:210-217(1997).
Lai F., Mol. Cell. Biol. 17:2413-2424(1997).
Villard L., Somat. Cell Mol. Genet. 23:135-145(1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01810 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, using ADARB1 antibody A01810.
Protein Target Info & Infographic
Gene/Protein Information For ADARB1 (Source: Uniprot.org, NCBI)
Gene Name
ADARB1
Full Name
Double-stranded RNA-specific editase 1
Weight
80763 MW
Alternative Names
Double-stranded RNA-specific editase 1;3.5.4.37;RNA-editing deaminase 1;RNA-editing enzyme 1;dsRNA adenosine deaminase;ADARB1;ADAR2, DRADA2, RED1; ADARB1 ADAR2, DRABA2, DRADA2, NEDHYMS, RED1 adenosine deaminase RNA specific B1 double-stranded RNA-specific editase 1|RNA editing deaminase 1|RNA-editing enzyme 1|adenosine deaminase, RNA-specific, B1 (homolog of rat RED1)|dsRNA adenosine deaminase DRADA2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADARB1, check out the ADARB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADARB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADARB1/Red1 Antibody (A01810)
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Customer Q&As
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4 Customer Q&As for Anti-ADARB1/Red1 Antibody
Question
I was wanting to use your anti-ADARB1/Red1 antibody for WB for mouse fetal brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse fetal brain identification?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
As indicated on the product datasheet, A01810 anti-ADARB1/Red1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-27
Question
Is this A01810 anti-ADARB1/Red1 antibody reactive to the isotypes of ADARB1?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The immunogen of A01810 anti-ADARB1/Red1 antibody is Synthesized peptide derived from internal of human ADARB1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-06
Question
We are currently using anti-ADARB1/Red1 antibody A01810 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
The anti-ADARB1/Red1 antibody (A01810) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-21
Question
I have a question about product A01810, anti-ADARB1/Red1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01810 anti-ADARB1/Red1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-14