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Product Info Summary
| SKU: | M01691 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody
SKU/Catalog Number
M01691
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody catalog # M01691. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01691)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOAO-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 67kDa Laminin Receptor
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01691 is reactive to RPSA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
v
Calculated molecular weight
32854 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01691 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of 67kDa Laminin Receptor using anti-67kDa Laminin Receptor antibody (M01691).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human COLO320 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-67kDa Laminin Receptor antigen affinity purified monoclonal antibody (Catalog # M01691) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 67kDa Laminin Receptor at approximately 40 kDa. The expected band size for 67kDa Laminin Receptor is at 33 kDa.
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Western blot analysis of 67kDa Laminin Receptor expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RPSA (Source: Uniprot.org, NCBI)
Gene Name
RPSA
Full Name
40S ribosomal protein SA
Weight
32854 MW
Superfamily
universal ribosomal protein uS2 family
Alternative Names
40S ribosomal protein SA ;37 kDa laminin receptor precursor ;37LRP ;37/67 kDa laminin receptor ;LRP/LR ;67 kDa laminin receptor ;67LR ;Colon carcinoma laminin-binding protein;Laminin receptor 1 ;LamR ;Laminin-binding protein precursor p40 ;LBP/p40 ;Multidrug resistance-associated protein MGr1-Ag;NEM/1CHD4;RPSA ;LAMBR, LAMR1; RPSA 37LRP, 67LR, ICAS, LAMBR, LAMR1, LBP, LBP/p40, LRP, LRP/LR, NEM/1CHD4, SA, lamR, p40 ribosomal protein SA 40S ribosomal protein SA|37 kDa laminin receptor|37/67 kDa laminin receptor|67 kDa laminin receptor|colon carcinoma laminin-binding protein|laminin receptor 1 (67kD, ribosomal protein SA)|laminin-binding protein precursor p40|multidrug resistance-associated protein MGr1-Ag|small ribosomal subunit protein uS2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPSA, check out the RPSA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPSA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody (M01691)
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