Anti-LRFN5 Antibody

Product Name

Anti-LRFN5 Antibody

View all LRFN5 Antibodies

SKU/Catalog Number

A12727

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LRFN5 Antibody (Catalog # A12727). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LRFN5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LRFN5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12727)

Host

Rabbit

Contents

LRFN5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LRFN5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LRFN5. The immunogen is located within amino acids 650 - 700 of LRFN5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of LRFN5 are known to exist. This antibody is predicted to not cross-react with other members of the LRFN family.

Reactive Species

A12727 is reactive to LRFN5 in Human, Mouse, Rat

Applications

A12727 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

79445 MW

Background of LRFN5

LRFN5 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while LRFN2 and LRFN5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LRFN5 antibody can be used for detection of LRFN5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of LRFN5 in EL4 cell lysate with LRFN5 antibody at (A) 0.5 and (B) 1 μg/mL.

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Immunohistochemistry of LRFN5 in mouse brain tissue with LRFN5 antibody at 5 μg/mL.

Gene/Protein Information For LRFN5 (Source: Uniprot.org, NCBI)

Alternative Names

C14orf146; chromosome 14 open reading frame 146; DKFZp686G0210; fibronectin type III, immunoglobulin and leucine rich repeat domains 8; FIGLER8; leucine rich repeat and fibronectin type III domain containing 5; leucine-rich repeat and fibronectin type-III domain-containing protein 5; LRFN5; SALM5; SALM5FLJ30803 LRFN5 C14orf146, FIGLER8, SALM5 leucine rich repeat and fibronectin type III domain containing 5 leucine-rich repeat and fibronectin type-III domain-containing protein 5|fibronectin type III, immunoglobulin and leucine rich repeat domains 8

For more info on LRFN5, check out the LRFN5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LRFN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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