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Product Info Summary
| SKU: | A02527-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-2B4/Cd244 Antibody Picoband®
View all 2B4/CD244/SLAMF4 Antibodies
SKU/Catalog Number
A02527-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-2B4/Cd244 Antibody Picoband® catalog # A02527-1. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-2B4/Cd244 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02527-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse 2B4/Cd244 recombinant protein (Position: Q20-N374).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02527-1 is reactive to Cd244 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
87164 MW
Background of 2B4/CD244/SLAMF4
CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al. (1999) mapped the 2B4 gene to 1q22. Suzuki et al. (2008) identified a functional single-nucleotide polymorphism (SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al. (1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al. (1998) demonstrated that CD48 binds to 2B4. Watzl et al. (2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02527-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
"Western blot, 0.25-0.5μg/ml, Mouse
ELISA, 0.1-0.5μg/ml, -
"
Positive Control
WB: mouse spleen tissue, mouse thymus tissue, mouse RAW2467 whole cell, mouse SP20 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Cd244 using anti-Cd244 antibody (A02527-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse RAW246.7 whole cell lysates,
Lane 3: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd244 antigen affinity purified polyclonal antibody (Catalog # A02527-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd244 at approximately 50KD. The expected band size for Cd244 is at 42KD.
Protein Target Info & Infographic
Gene/Protein Information For Cd244 (Source: Uniprot.org, NCBI)
Gene Name
Cd244
Full Name
Natural killer cell receptor 2B4
Weight
87164 MW
Alternative Names
Natural killer cell receptor 2B4; NK cell type I receptor protein 2B4; NKR2B4; Non-MHC restricted killing associated; SLAM family; SLAMF4; Signaling lymphocytic activation molecule 4; CD244; Cd244; 2b4, Nmrk CD244 2B4, NAIL, NKR2B4, Nmrk, SLAMF4 CD244 molecule natural killer cell receptor 2B4|CD244 molecule, natural killer cell receptor 2B4|NK cell activation inducing ligand NAIL|NK cell activation-inducing ligand|NK cell type I receptor protein 2B4|SLAM family member 4|h2B4|signaling lymphocytic activation molecule 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd244, check out the Cd244 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd244: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-2B4/Cd244 Antibody Picoband® (A02527-1)
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