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Product Info Summary
| SKU: | EKC1960 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Smad3 (Phospho-Ser425) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1960
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Smad3 (Phospho-Ser425) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Smad3 (Phospho-Ser425) protein expression profile in cells. The kit can be used for measuring the relative amounts of Smad3 (Phospho-Ser425) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Smad3 (Phospho-Ser425).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Smad3 (Phospho-Ser425) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1960)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1960 is reactive to SMAD3 in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1960 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Smad3 Phospho-Ser425 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Smad3 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from NIH-3T3 cells treated with IGF-I 100ng/ml 10'
Protein Target Info & Infographic
Gene/Protein Information For SMAD3 (Source: Uniprot.Org, NCBI)
Gene Name
SMAD3
Full Name
Mothers against decapentaplegic homolog 3
Weight
48081 MW
Superfamily
dwarfin/SMAD family
Alternative Names
JV15-2;MAD-3;MADH3;Mad3;Mothers against DPP homolog 3;Mothers against decapentaplegic homolog 3;SMAD 3;Smad 3 SMAD3 HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3 SMAD family member 3 mothers against decapentaplegic homolog 3|MAD homolog 3|MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|hMAD-3|hSMAD3|mad homolog JV15-2|mad protein homolog|mad3|mothers against DPP homolog 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SMAD3, check out the SMAD3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SMAD3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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