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Key=entity_id, value=53846
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Key=type_id, value=simple
Key=sku, value=EKC1410
Key=has_options, value=0
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Key=updated_at, value=2024-09-26 01:01:25
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Key=size, value=1 kit, containing one 96-well plate and all necessary reagents
Key=gene_full_name, value=Nuclear receptor subfamily 4 group A member 1
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Key=assay_range, value=> 5000 cells/well
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Key=description, value=The Nuclear Receptor NR4A1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Nuclear Receptor NR4A1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Nuclear Receptor NR4A1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Nuclear Receptor NR4A1.
Key=short_description, value=The Nuclear Receptor NR4A1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Nuclear Receptor NR4A1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Nuclear Receptor NR4A1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Nuclear Receptor NR4A1.
Key=gene_name, value=NR4A1
Key=synonyms, value=Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3;NR4A1;GFRP1, HMR, NAK1;
Key=kit_components, value=Nuclear Receptor NR4A1
Key=subcellular_localization, value=Nucleus. Cytoplasm. Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27.
Key=tissue_specificity, value= Fetal muscle and adult liver, brain and thyroid.
Key=protein_function, value= Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'- AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury (By similarity).
Key=storage, value=Store at 4°C for up to 6 months.
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Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit
NGFI-B alpha/Nur77/NR4A1 ELISA kit, cell-based
The Nuclear Receptor NR4A1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Nuclear Receptor NR4A1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Nuclear Receptor NR4A1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Nuclear Receptor NR4A1.
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Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit Validation Images Gallery
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Product Info Summary
SKU:
EKC1410
Size:
1 kit, containing one 96-well plate and all necessary reagents
Reactive Species:
Human, Mouse, Rat
Application:
ELISA
Product Name
Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit
See all NGFI-B alpha/Nur77/NR4A1 products
SKU/Catalog Number
EKC1410
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The Nuclear Receptor NR4A1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Nuclear Receptor NR4A1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Nuclear Receptor NR4A1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Nuclear Receptor NR4A1.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1410)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1410 is reactive to NR4A1 in Human, Mouse, Rat samples
Application Guarantee
EKC1410 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent
Quantity
Container
96-Well Cell Culture Clear-Bottom Microplate
1 Plates
-
10x TBS
24 ml (10x)
Clear
Quenching Buffer
24 ml (1x)
Clear
Blocking Buffer
50 ml (1x)
Clear
15x Wash Buffer
50 ml (15x)
Clear
100x Anti-Nuclear Receptor NR4A1 Antibody (Rabbit Polyclonal)
60 µl (100x)
Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal)
60 µl (100x)
Green
HRP-Conjugated Anti-Rabbit IgG Antibody
6ml (1x)
Glass
HRP-Conjugated Anti-Mouse IgG Antibody
6ml (1x)
Glass
Primary Antibody Diluent
12 ml (1x)
Clear
Ready-to-Use Substrate
12 ml (1x)
Brown
Stop Solution
12 ml (1x)
Clear
Crystal Violet Solution
6ml (1x)
Glass
SDS Solution
24 ml (1x)
Clear
Adhesive Plate Seals
2 Seals
-
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional) Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources Deionized or sterile water Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer Graph paper or computer software capable of generating or displaying logarithmic functions Absorbent papers or vacuum aspirator Test tubes or microfuge tubes capable of storing 1 ml Orbital shaker Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Western blot analysis of extracts from COS7 cells
Gene/Protein Information For NR4A1 (Source: Uniprot.Org, NCBI)
Full Name
Nuclear receptor subfamily 4 group A member 1
Superfamily
nuclear hormone receptor family
Alternative Names
Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3;NR4A1;GFRP1, HMR, NAK1; NR4A1 GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3 nuclear receptor subfamily 4 group A member 1 nuclear receptor subfamily 4 group A member 1|ST-59|TR3 orphan receptor|early response protein NAK1|growth factor-inducible nuclear protein N10|hormone receptor|nerve growth factor IB nuclear receptor variant 1|nuclear hormone receptor NUR/77|orphan nuclear receptor HMR|orphan nuclear receptor TR3|steroid receptor TR3|testicular receptor 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NR4A1, check out the NR4A1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NR4A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected] .
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