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Key=description, value=The Nuclear Receptor NR4A1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Nuclear Receptor NR4A1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Nuclear Receptor NR4A1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Nuclear Receptor NR4A1.
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Key=gene_name, value=NR4A1
Key=synonyms, value=Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3;NR4A1;GFRP1, HMR, NAK1;
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Key=subcellular_localization, value=Nucleus. Cytoplasm. Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27.
Key=tissue_specificity, value= Fetal muscle and adult liver, brain and thyroid.
Key=protein_function, value= Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'- AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury (By similarity).
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Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit

NGFI-B alpha/Nur77/NR4A1 ELISA kit, cell-based

The Nuclear Receptor NR4A1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Nuclear Receptor NR4A1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Nuclear Receptor NR4A1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Nuclear Receptor NR4A1.

Product Info Summary

SKU: EKC1410
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit

See all NGFI-B alpha/Nur77/NR4A1 products

SKU/Catalog Number

EKC1410

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The Nuclear Receptor NR4A1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Nuclear Receptor NR4A1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Nuclear Receptor NR4A1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Nuclear Receptor NR4A1.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1410)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1410 is reactive to NR4A1 in Human, Mouse, Rat samples

Application Guarantee

EKC1410 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Nuclear Receptor NR4A1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For NR4A1 (Source: Uniprot.Org, NCBI)

Gene Name

NR4A1

Full Name

Nuclear receptor subfamily 4 group A member 1

Weight

64463 MW

Superfamily

nuclear hormone receptor family

Alternative Names

Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3;NR4A1;GFRP1, HMR, NAK1; NR4A1 GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3 nuclear receptor subfamily 4 group A member 1 nuclear receptor subfamily 4 group A member 1|ST-59|TR3 orphan receptor|early response protein NAK1|growth factor-inducible nuclear protein N10|hormone receptor|nerve growth factor IB nuclear receptor variant 1|nuclear hormone receptor NUR/77|orphan nuclear receptor HMR|orphan nuclear receptor TR3|steroid receptor TR3|testicular receptor 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NR4A1, check out the NR4A1 Infographic

NR4A1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NR4A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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