Array
(
[0] => store_id
[1] => entity_id
[2] => attribute_set_id
[3] => type_id
[4] => sku
[5] => has_options
[6] => required_options
[7] => created_at
[8] => updated_at
[9] => mst_search_weight
[10] => status
[11] => name
[12] => cj_search_index
[13] => publication_count
[14] => qty_in_warehouses
[15] => is_recurring
[16] => visibility
[17] => tax_class_id
[18] => featured
[19] => featured_image
[20] => fb_product
[21] => custom_product_page
[22] => free_shipping
[23] => template
[24] => host
[25] => quantity_and_stock_status
[26] => googleshopping_exclude
[27] => yoast_keyword_score
[28] => yoast_content_score
[29] => badge
[30] => price
[31] => weight
[32] => meta_title
[33] => meta_description
[34] => image
[35] => small_image
[36] => thumbnail
[37] => url_key
[38] => url_path
[39] => options_container
[40] => image_label
[41] => small_image_label
[42] => thumbnail_label
[43] => msrp_display_actual_price_type
[44] => gift_message_available
[45] => size
[46] => protein_name
[47] => gene_full_name
[48] => uniprot_id
[49] => reconstitution
[50] => sensitivity
[51] => applications
[52] => reactivity
[53] => form
[54] => concentration
[55] => cross_reactivity
[56] => recommended_detection_systems
[57] => predicted_reactivity
[58] => source_company
[59] => isotype
[60] => molecular_weight
[61] => sample_size_available
[62] => related_product_skus
[63] => product_category
[64] => research_category
[65] => clonality
[66] => swatch_image
[67] => rating_value
[68] => review_count
[69] => phospho_site
[70] => mp_exclude_sitemap
[71] => yoast_focus_keyword
[72] => yoast_facebook_image
[73] => yoast_twitter_image
[74] => cj_related_products
[75] => description
[76] => short_description
[77] => meta_keyword
[78] => contents
[79] => background
[80] => gene_name
[81] => synonyms
[82] => application_details
[83] => subcellular_localization
[84] => tissue_specificity
[85] => protein_function
[86] => application_notes
[87] => competitor_equivalent_skus
[88] => immunogen
[89] => purification
[90] => storage
[91] => custom_attribute_1
[92] => custom_attribute_2
[93] => custom_attribute_3
[94] => options
[95] => media_gallery
[96] => extension_attributes
[97] => tier_price
[98] => tier_price_changed
[99] => category_ids
[100] => is_salable
[101] => website_ids
[102] => request_path
[103] => rating_summary
[104] => _cache_instance_store_filter
[105] => media_gallery_images
)
Key=store_id Key=entity_id, value=3405 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=PB9766 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2015-11-24 15:33:59 Key=updated_at, value=2024-09-26 00:46:55 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-NUR77/NR4A1 Antibody Picoband® Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1365 Key=yoast_content_score, value=60 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-NUR77/NR4A1 Antibody Picoband® | Bosterbio Key=meta_description, value=Boster Bio Anti-NUR77/NR4A1 Antibody Picoband® catalog # PB9766. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody. Key=image, value=/p/b/pb9766-1.jpg Key=small_image, value=/p/b/pb9766-1.jpg Key=thumbnail, value=/p/b/pb9766-1.jpg Key=url_key, value=anti-nur77-picoband-trade-antibody-pb9766-boster Key=url_path, value=anti-nur77-picoband-trade-antibody-pb9766-boster Key=options_container, value=Product Info Column Key=image_label, value=Anti- NUR77 Picoband antibody, PB9766, Western blotting All lanes: Anti NUR77 (PB9766) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: HELA Whol Key=small_image_label, value=Anti- NUR77 Picoband antibody, PB9766, Western blotting All lanes: Anti NUR77 (PB9766) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: HELA Whol Key=thumbnail_label, value=Anti- NUR77 Picoband antibody, PB9766, Western blotting All lanes: Anti NUR77 (PB9766) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: HELA Whol Key=msrp_display_actual_price_type, value= Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=protein_name, value=Nuclear receptor subfamily 4 group A member 1 Key=gene_full_name, value=Nuclear receptor subfamily 4 group A member 1 Key=uniprot_id, value=P22736 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Key=sensitivity Key=applications, value=WB Key=reactivity, value=Human, Mouse, Rat Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins. Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Key=predicted_reactivity Key=source_company, value=Boster PB9766 Key=isotype, value=Rabbit IgG Key=molecular_weight, value=64463 MW Key=sample_size_available, value=30ug for $99, contact us for details Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=Apoptosis, Cancer, Cell Biology, Cell Death, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Invasion/Microenvironment, Nuclear Receptors, Nuclear Signaling Pathways, Nucleus Key=clonality, value=Polyclonal Key=swatch_image, value=/e/k/ek1402_1.png Key=rating_value, value=98.9 Key=review_count, value=12 Key=phospho_site Key=mp_exclude_sitemap, value=Yes Key=yoast_focus_keyword, value=CD68 antibody Key=yoast_facebook_image, value=/p/b/pb9568-ahsg-primary-antibodies-wb-testing-1.jpg Key=yoast_twitter_image, value=/p/b/pb9568-ahsg-primary-antibodies-wb-testing-1.jpg Key=cj_related_products, value=Atherosclerosis:FEK0370^Schizophrenia:A14829^Vascular Diseases:A00557-2|EK1646|A02851|A02851-1 Key=description, value=Boster Bio Anti-NUR77/NR4A1 Antibody Picoband® catalog # PB9766. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-NUR77/NR4A1 Antibody Picoband® catalog # PB9766. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-NUR77/NR4A1 Antibody Picoband® Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Key=background, value=NR4A1 (NUCLEAR RECEPTOR SUBFAMILY 4, GROUP A, MEMBER 1), also called NAK1, GFRP1, TR3, NUR77 or NGFIB, is a protein that in humans is encoded by the NR4A1 gene, which is also a member of the Nur nuclear receptor family of intracellular transcription factors. The NR4A1 gene is mapped on 12q13.13. NR4A1 is involved in cell cycle mediation, inflammation and apoptosis. It plays a key role in mediating inflammatory responses in macrophages. In addition, subcellular localization of the NR4A1 protein appears to play a key role in the survival and death of cells. Nr4a1 is overexpressed in Wnt1 -transformed mouse mammary cells. Nr4a1 is also induced by lithium, a Wnt1 mimic, and the Nr4a1 promoter is activated by lithium and beta-catenin, a Wnt1 downstream effector. In contrast, human NR4A1 is not upregulated by beta-catenin, indicating that this gene is regulated differently in human and mouse cells. Adenoviral expression of Nr4a1 induces genes involved in gluconeogenesis, stimulates glucose production both in vitro and in vivo, and raises blood glucose levels. Key=gene_name, value=NR4A1 Key=synonyms, value=Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3;NR4A1;GFRP1, HMR, NAK1; Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Key=subcellular_localization, value= Cytoplasm. Nucleus. Key=tissue_specificity, value= Fetal muscle and adult liver, brain and thyroid. Key=protein_function, value= Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'- AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. . Key=application_notes, value=Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 16991|sc 16991 R|sc 16992|sc 16992 R|sc 365113|sc 365113 X|sc 5569|sc 5569 X|sc 7013|sc 7013 X|sc 7014|sc 7014 X|sc 81344|sc 990|sc 990 X Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human NUR77, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. Key=purification, value=Immunogen affinity purified. Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=67 kDa Key=custom_attribute_2, value=WB: Rat Brain Tissue, Mouse Brain Tissue, U87 Whole Cell, HELA Whole Cell Key=custom_attribute_3, value=PB0818 Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-NUR77/NR4A1 Antibody Picoband®
NGFI-B alpha/Nur77/NR4A1 antibody
Boster Bio Anti-NUR77/NR4A1 Antibody Picoband® catalog # PB9766. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).
Boster Bio Anti-NUR77/NR4A1 Antibody Picoband® catalog # PB9766. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NUR77/NR4A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9766)
A synthetic peptide corresponding to a sequence at the C-terminus of human NUR77, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9766 is reactive to NR4A1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
67 kDa
Calculated molecular weight
64463 MW
Background of NGFI-B alpha/Nur77/NR4A1
NR4A1 (NUCLEAR RECEPTOR SUBFAMILY 4, GROUP A, MEMBER 1), also called NAK1, GFRP1, TR3, NUR77 or NGFIB, is a protein that in humans is encoded by the NR4A1 gene, which is also a member of the Nur nuclear receptor family of intracellular transcription factors. The NR4A1 gene is mapped on 12q13.13. NR4A1 is involved in cell cycle mediation, inflammation and apoptosis. It plays a key role in mediating inflammatory responses in macrophages. In addition, subcellular localization of the NR4A1 protein appears to play a key role in the survival and death of cells. Nr4a1 is overexpressed in Wnt1 -transformed mouse mammary cells. Nr4a1 is also induced by lithium, a Wnt1 mimic, and the Nr4a1 promoter is activated by lithium and beta-catenin, a Wnt1 downstream effector. In contrast, human NR4A1 is not upregulated by beta-catenin, indicating that this gene is regulated differently in human and mouse cells. Adenoviral expression of Nr4a1 induces genes involved in gluconeogenesis, stimulates glucose production both in vitro and in vivo, and raises blood glucose levels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, U87 Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NUR77 using anti-NUR77 antibody (PB9766).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: U87 Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUR77 antigen affinity purified polyclonal antibody (Catalog # PB9766) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUR77 at approximately 67 kDa. The expected band size for NUR77 is at 67 kDa.
Gene/Protein Information For NR4A1 (Source: Uniprot.org, NCBI)
Gene Name
NR4A1
Full Name
Nuclear receptor subfamily 4 group A member 1
Weight
64463 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3;NR4A1;GFRP1, HMR, NAK1; NR4A1 GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3 nuclear receptor subfamily 4 group A member 1 nuclear receptor subfamily 4 group A member 1|ST-59|TR3 orphan receptor|early response protein NAK1|growth factor-inducible nuclear protein N10|hormone receptor|nerve growth factor IB nuclear receptor variant 1|nuclear hormone receptor NUR/77|orphan nuclear receptor HMR|orphan nuclear receptor TR3|steroid receptor TR3|testicular receptor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on NR4A1, check out the NR4A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
NR4A Nuclear Receptors Target Poly-ADP-Ribosylated DNA-PKcs Protein to Promote DNA Repair
1 Customer Q&As for Anti-NUR77/NR4A1 Antibody Picoband®
Question
We are currently using anti-NUR77/NR4A1 antibody PB9766 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-NUR77/NR4A1 antibody (PB9766) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.