NR4A1 Antibodies

Nuclear receptor subfamily 4 group A member 1 (NR4A1)

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration.

Binds the NGFI-B response element (NBRE) 5'- AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.

Gene Information

Human
Gene Name:	NR4A1
Uniprot:	P22736
Entrez:	3164

Family

Belongs to:
nuclear hormone receptor family

Alternative Names

Early response protein NAK1; GFRP1ST-59; growth factor-inducible nuclear protein N10; HMR; HMRNP10; hormone receptor; MGC9485; N10; NAK1; NAK-1; NGFIB alpha; NGFI-B alpha; NGFIB; NR4A1; Nuclear hormone receptor NUR/77; nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; steroid receptor TR3; Testicular receptor 3; TR3 orphan receptor; TR3

Molecular Weight

Mass (kDA):
64.463 kDA

Genomic Context

Human
Location:	12q13.13
Sequence:	12; NC_000012.12 (52022832..52059507)

Tissue Specificity

Fetal muscle and adult liver, brain and thyroid.

Subcellular Localizations

Nucleus. Cytoplasm. Mitochondrion. Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340). Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950).

Diseases

• Malignant Neoplasms
• Neoplasms
• Inflammation
• Pituitary Diseases
• Leukemia
• Atherosclerosis
• Carcinoma
• Nervousness
• Lymphoma
• Physiological Stress
• Malignant Neoplasm Of Breast
• Lung Neoplasms

• Schizophrenia
• Obesity
• Growth Arrest
• Mammary Neoplasms
• Liver Neoplasms
• Cell Transformation, Neoplastic
• Vascular Diseases

Interacting Proteins

• ANKIB1
• BCL2
• BNIP3L
• CDKN2D
• CUTA

• GK
• KRTAP10-5
• LONRF1
• MATR3
• PGBD1

• PLXND1
• PRMT1
• RELA
• SLC25A4
• STAT3

• TP53
• TUBB4A
• VASP

Pathways

• Cell Death
• Cell Cycle
• Localization
• Cell Proliferation
• Secretion
• Cell Growth
• Induction Of Apoptosis
• Nuclear Export
• Pathogenesis
• Rna Interference
• Programmed Cell Death
• Cell Development
• Gluconeogenesis

• Transport
• Immune Response
• Regulation Of Gene Expression
• Inflammatory Response
• Neuroprotection
• Cell Cycle Arrest
• Cell Activation

PTMs

• Phosphorylation
• Cleavage
• Acetylation
• Oxidation
• Methylation
• Ubiquitination

• Demethylation
• Deacetylation
• Dephosphorylation
• Biotinylation
• Carboxylation
• Myristoylation

Research Areas

• Apoptosis

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NR4A1

What Are The Best Uses Of The NR4A1 Marker?

What are the best uses of the NR4A1, N4A2, and NR4A3 Markers? This article will provide answers to these questions and more. It also applies to all scientists from around the world. What exactly is NR4A1? And how can it be used to know the genome of your nematode? Let's take a look at some of the best applications of this marker for protein.

NR4A1

In vitro decidualization is an underlying biological process that causes cells to express NR4A1 genes. NR4A1 appears to play a significant role among the three NR4A-receptors. Studies on molecular biology are being conducted to determine whether NR4A1 is involved in decidualization. Boster Bio provides the NR4A1 marker.

FOXO1A is an transcription factor that plays a role in NR4A decidualization that is induced by NR4A. NR4A1 direct targets FOXO1A. The PCR products were isolated by electrophoresis of agarose gels. As as a positive control input chromatin was utilized. HESCs were infected for 24 hrs with the indicated Adenoviruses , and then transfected using 500 ng firefly reporter or luciferase reporter RNAs. After 48 hours Luciferase assays were conducted. The Renilla Luciferase level was normalized to levels of Luciferase.

NR4A1 is expressed in human endometrial tissues. NR4A regulates decidualization and upregulates prolactin, insulin-like growth factor binding proteins-1, and regulates decidualization. It is implicated in female subfertility. Loss of uterine Nr4A1 in women can cause female subfertility. Inhibition of IGFBP-1 or PRL expression due to the absence of NR4A1 can be caused by the ovarian tissue being deficient in NR4A1.

NR4A2

We have identified a miRNA which targets NR4A2 in Boster Bio. The miRNAs target the 3' UTR of the NR4A2. The luminescence reporter signal is reduced by the miRNAs. Both miRNAs target NR4A2, but they do this through different pathways. We have identified which miRNAs target the NR4A2 via miRNA screening as a modeling tool.

The NR4A2 protein is an essential one in the gastric adenocarcinoma cellular cells. The protein is involved in cell-mediated responses and is regulated in gastric adenocarcinoma tumor cells. The experiments found that NR4A2 knockdown cells increased their migration by a number of times between two to four. The results demonstrate that gastrin is able to increase cell movement by increasing the speed of NR4A2 knockdown.

The transient treatment of AGS-GR cells with NR4A2-EGFP was carried out 24 hours after the serum-starved treatment. The secondary antibody was detected using Kodak Image Station 2000R. Antibodies used in this study were from Santa Cruz Biotechnology and Abcam. Monoclonal to beta-actin antibody from Abcam was utilized. Polyclonal HRP goat anti-mouse IgG from Dako, Glostrup, Denmark was used to observe the cell death of NR4A2.

NBRE-mediated activation can be mediated by NR4A2. Inhibitors of gastrin and the PKA/PKC signaling pathways block gastrin-induced NR4A2 gene expression. These inhibitors aren't specific for NR4A2 but they do block gastrin-induced NBRE-luc gene expression. Gastrin-induced NBRE also phosphorylates PKB/Akt.

NR4A2 is a nuclear receptor subfamily 4 member 2 gene that regulates gene expression. This is the sole reason behind NR4A2 expression in Boster Bio antibody. The cells that score the highest are derived from the first two postnatal stages. The more points scored is the more specific reagent. Boster Bio is a great source for these molecules.

NR4A3

The posttranslational modification protein SMARCB1 regulates NR4A3 expression in boster bio. This modification, mediated by ROS, is critical for NR4A3 degradation in VEC. ROS-induced downregulation in the NR4A3 gene is lowered by antioxidants NAC (and TRIOL).

Transgenic mice carrying AP2-NR4A3 had no difference in the levels of serum leptin compared to wild-type littermates. Additionally their serum catecholamine levels were significantly decreased, whereas those of their wild-type littermates did not. These results suggest that mice with NR4A3 deficiency have reduced glucose tolerance and insulin resistance. However, many other studies haven't yet been conducted in this mouse strain.

Gal4-based yeast two hybrid screening was utilized to amplify the NR4A3 gene according to the protocol of the manufacturer. A PCR product encoding NR4A3-C (308 amino acid fragment) was created in the Sfi I site of the pGBKT7 genome. Additionally to this, a human fetal brain cDNA library has been purchased from Clontech. It was subcloned into the pACT2 vector and transfected into Y190 to form a pool of prey. DNA sequencing and BLASTn searches were used to identify positive clones.

NR4A3 is found in many cells of different types. It regulates the inflammatory response by preventing the activation of NF-kB. The overexpression of NR4A3 in the pulmonary endothelium reduces the upregulation caused by LPS of cytokines. The siRNA knockdown of NR4A3 resulted in increased levels of pro-inflammatory mediators in the smooth muscle cells of the vascular system.

RNAi-shNR4A3 (RBS) particles are designed to block the expression of the human NR4A3 gene. The particles were introduced into chondrocytes by using Lipofectamine 2000 transfection reagent. The cells were then placed incubated at room temperature for either one hour, two hours or three hours. The results of these experiments demonstrate that NR4A3 overexpression enhances the expression of chondrocytes.

Scientists can do many studies using this antibody against NR4A3. They can test NR4A3 expression in a variety of cell lines and present results related to the application. Additionally, they may earn product credits. This method is applicable to scientists from any country. The NR4A3 gene is involved in several cell processes. Boster Bio Anti-NR4A3 antibody may be used to analyze various biological samples.

NR4A3 induces the expression of catecholamine degrading enzymes. This decreases levels of epinephrine the blood. Low levels of epinephrine can cause an increase in LDL and impaired glucose tolerance. The protein has also been linked with reduced cardiac activity and LV dilation. These findings underscore the importance of NR4A3 in regulating blood sugar levels.

NR4A3 is highly expressed in OA cartilage. Its overexpression in cartilage with OA-related ossification is associated with decreased expression of COX-2, an important marker of the disease. In addition, knockdowns to NR4A3 resulted in a decrease in IL-1b levels, a well-known pro-inflammatory mediator. By knocking out NR4A3 in chondrocytes, apoptosis-induced cell death was also reduced.