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Product Info Summary
| SKU: | EK0706-PR |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Monkey |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Monkey primate CD56/NCAM-1 ELISA Kit PicoKine®
View all NCAM-1/CD56 ELISA kits
SKU/Catalog Number
EK0706-PR
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Monkey primate CD56/NCAM-1 ELISA Kit PicoKine® (96 Tests). Quantitate Monkey NCAM1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Monkey primate CD56/NCAM-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0706-PR)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L20-G718
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0706-PR for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0706-PR is reactive to NCAM1 in Monkey samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0706-PR is guaranteed for ELISA in Monkey by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of NCAM-1/CD56
Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0706-PR-CAP | Anti-Monkey NCAM1 Pre-coated 96-well strip microplate | 1 |
| EK0706-PR-ST | Monkey NCAM1 Standard | 2 vials, 10 ng/tube |
| EK0706-PR-DA | Monkey NCAM1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10000 |
| O.D. | 0.019 | 0.152 | 0.286 | 0.494 | 0.865 | 1.493 | 2.100 | 2.411 |
Data Example Images
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Monkey Primate CD56/NCAM-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Monkey primate CD56/NCAM-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml) | 1.52 | 3.48 | 5.34 | 2.06 | 4.16 | 6.27 |
| Standard deviation | 0.082 | 0.212 | 0.368 | 0.140 | 0.312 | 0.508 |
| CV (%) | 5.4 | 6.1 | 6.9 | 6.8 | 7.5 | 8.1 |
Protein Target Info & Infographic
Gene/Protein Information For NCAM1 (Source: Uniprot.Org, NCBI)
Gene Name
NCAM1
Full Name
Neural cell adhesion molecule 1
Weight
94574 MW
Alternative Names
Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56;NCAM1;NCAM; NCAM1 CD56, MSK39, NCAM neural cell adhesion molecule 1 neural cell adhesion molecule 1| recognized by 5.1H11|neural cell adhesion molecule, NCAM
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NCAM1, check out the NCAM1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NCAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Monkey primate CD56/NCAM-1 ELISA Kit PicoKine® (EK0706-PR)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Monkey primate CD56/NCAM-1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-23
Question
Q: What is the optimal O.D. value for CD56 ELISA kit? I used your CD56 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD56 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD56 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD56 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-04-03
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CD56 in Monkey plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
D. Singh
Verified customer
Asked: 2018-10-26
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD56 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-26
Question
Q: Can CD56 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
H. Thomas
Verified customer
Asked: 2018-02-05
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-02-05
Question
Q: how to proceed with the analysis of ELISA data? I measured CD56 level in plasma.
Verified Customer
Verified customer
Asked: 2016-10-09
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-10-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-10-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-10-16
