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Product Info Summary
| SKU: | EK0803 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine®
View all XCL1/Lymphotactin ELISA kits
SKU/Catalog Number
EK0803
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Xcl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0803)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V22-G114
Sensitivity
<5 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0803 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0803 is reactive to Xcl1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0803 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of XCL1/Lymphotactin
Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0803-CAP | Anti-Mouse Xcl1 Pre-coated 96-well strip microplate | 1 |
| EK0803-ST | Mouse Xcl1 Standard | 2 vials, 10 ng/tube |
| EK0803-DA | Mouse Xcl1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.031 | 0.203 | 0.390 | 0.574 | 0.969 | 1.409 | 1.689 | 2.064 |
Data Example Images
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Mouse XCL1/Lymphotactin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 209 | 665 | 1909 | 199 | 711 | 1727 |
| Standard deviation | 8.56 | 34.58 | 114.54 | 8.55 | 49.77 | 112.25 |
| CV (%) | 4.1% | 5.2% | 6% | 4.3% | 7% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of XCL1/Lymphotactin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 209 | 185 | 189 | 201 | 196 | 9.53 | 4.8% |
| Sample 2 | 665 | 691 | 673 | 711 | 685 | 17.72 | 2.5% |
| Sample 3 | 1909 | 1945 | 1858 | 1909 | 1905 | 30.98 | 1.6% |
Protein Target Info & Infographic
Gene/Protein Information For Xcl1 (Source: Uniprot.Org, NCBI)
Gene Name
Xcl1
Full Name
Lymphotactin
Weight
12467 MW
Superfamily
intercrine gamma family
Alternative Names
Lymphotactin;C motif chemokine 1;Cytokine SCM-1;Lymphotaxin;Small-inducible cytokine C1;Xcl1;Lptn, Ltn, Scyc1; XCL1 ATAC, LPTN, LTN, SCM-1, SCM-1a, SCM1, SCM1A, SCYC1 X-C motif chemokine ligand 1 lymphotactin|SCM-1-alpha|XC chemokine ligand 1|c motif chemokine 1|chemokine (C motif) ligand 1|cytokine SCM-1|lymphotaxin|single cysteine motif 1a|small inducible cytokine subfamily C, member 1 (lymphotactin)|small-inducible cytokine C1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Xcl1, check out the Xcl1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Xcl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine® (EK0803)
Hello CJ!
EK0803 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The systemic exercise-released chemokine lymphotactin/XCL1 modulates in vitro adult hippocampal precursor cell proliferation and neuronal differentiation
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine®
Question
Does EK0803 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine® (EK0803) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-06-24
Question
Q: is it okay to use citrate plasma as samples in Mouse XCL1 Picokine® ELISA Kit (Catalog # EK0803)?
E. Yang
Verified customer
Asked: 2019-03-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of XCL1 causing degradation of its protein structure. XCL1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the XCL1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-03-18
Question
Q: What is the optimal O.D. value for XCL1 ELISA kit? I used your XCL1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain XCL1 even though the O.D. values are not very high?
K. Walker
Verified customer
Asked: 2018-09-22
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this XCL1 ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of XCL1 that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2018-09-22
Question
Q: if the enzyme conjugated XCL1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the XCL1 antigen?
Verified Customer
Verified customer
Asked: 2018-04-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-04-01
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement XCL1 using the EK0803 Mouse XCL1 Picokine® ELISA Kit?
W. Babu
Verified customer
Asked: 2018-03-15
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-03-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Zhou
Verified customer
Asked: 2014-03-15
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-03-15
