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Product Info Summary
| SKU: | A03237-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-CCL8 Antibody
View all CCL8/MCP-2 Antibodies
SKU/Catalog Number
A03237-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCL8 Antibody catalog # A03237-1. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCL8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03237-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CCL8 recombinant protein (Position: G24-P97).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03237-1 is reactive to Ccl8 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
71 kDa
Calculated molecular weight
11.246kDa
Background of CCL8/MCP-2
CCL8, also known as monocyte chemoattractant protein 2 (MCP2), is a protein that in humans is encoded by the CCL8 gene. It is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B, CCR3 and CCR5.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03237-1 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
IHC: mouse kidney tissue, mouse small intestine tissue, mouse spleen tissue, rat kidney tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of CCL8 using anti-CCL8 antibody (A03237-1).
CCL8 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL8 Antibody (A03237-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of CCL8 using anti-CCL8 antibody (A03237-1).
CCL8 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL8 Antibody (A03237-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CCL8 using anti-CCL8 antibody (A03237-1).
CCL8 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL8 Antibody (A03237-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CCL8 using anti-CCL8 antibody (A03237-1).
CCL8 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL8 Antibody (A03237-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ccl8 (Source: Uniprot.org, NCBI)
Gene Name
Ccl8
Full Name
C-C motif chemokine 8
Weight
11.246kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 8; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8; Ccl8; Mcp2; Scya8 CCL8 HC14, MCP-2, MCP2, SCYA10, SCYA8 C-C motif chemokine ligand 8 C-C motif chemokine 8|chemokine (C-C motif) ligand 8|monocyte chemoattractant protein 2|monocyte chemotactic protein 2|small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)|small-inducible cytokine A8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccl8, check out the Ccl8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccl8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCL8 Antibody (A03237-1)
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Customer Q&As
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7 Customer Q&As for Anti-CCL8 Antibody
Question
I was wanting to use your anti-CCL8 antibody for IHC for mouse bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse bone marrow identification?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
You can see on the product datasheet, A03237-1 anti-CCL8 antibody has been tested for ELISA, IHC on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-16
Question
Is this A03237-1 anti-CCL8 antibody reactive to the isotypes of CCL8?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The immunogen of A03237-1 anti-CCL8 antibody is E. coli-derived mouse CCL8 recombinant protein (Position: G24-P97). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-13
Question
We are currently using anti-CCL8 antibody A03237-1 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
The anti-CCL8 antibody (A03237-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-CCL8 antibody A03237-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-05
Question
Does anti-CCL8 antibody A03237-1 work for IHC with bone marrow?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
According to the expression profile of bone marrow, CCL8 is highly expressed in bone marrow. So, it is likely that anti-CCL8 antibody A03237-1 will work for IHC with bone marrow.
Boster Scientific Support
Answered: 2017-10-02
Question
I see that the anti-CCL8 antibody A03237-1 works with IHC, what is the protocol used to produce the result images on the product page?
T. Moore
Verified customer
Asked: 2017-05-18
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-18
Question
I have a question about product A03237-1, anti-CCL8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Huang
Verified customer
Asked: 2016-03-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03237-1 anti-CCL8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-03-04
