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Key=size, value=96 wells/kit, with removable strips.
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Key=description, value=Mouse TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Mouse TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
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Key=background, value=Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.
Key=gene_name, value=Tnfrsf25
Key=synonyms, value=Protein Tnfrsf25 ;Tumor necrosis factor receptor superfamily, member 25, isoform CRA_b ;Tnfrsf25 ;mCG_4090 ;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=10 ng/tube
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  • Microplate reader in standard size.
  • Automated plate washer.
  • Incubator.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

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Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2061289433220112933972
Standard deviation8.4468.31316.2311.6568.52317.76
CV (%)4.1%5.3%7.3%5.8%5.3%8%

Key=application_details, value=Dilution ratio of 1:1, concentration in serum and plasma is around 1000 pg/ml.
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Concentration (pg/ml)015631262512500025000500010000
O.D.0.0410.0920.1220.1950.4010.8471.7412.402

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Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: Q31-F199
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120620922218920611.755.7%
Sample 21289116312091152120353.924.4%
Sample 343324412405446544363214.194.9%
*number of samples for each test n=16.
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Mouse TNFRSF25/DR3 ELISA Kit PicoKine®

DR3/TNFRSF25 ELISA kit for Mouse

Mouse TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK1515
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse TNFRSF25/DR3 ELISA Kit PicoKine®

View all DR3/TNFRSF25 ELISA kits

SKU/Catalog Number

EK1515

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse TNFRSF25/DR3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1515)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q31-F199

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK1515 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1515 is reactive to Tnfrsf25 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1515 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of DR3/TNFRSF25

Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

Kit Components

Catalog Number Description Quantity
EK1515-CAP Anti-Mouse Tnfrsf25 Pre-coated 96-well strip microplate 1
EK1515-ST Mouse Tnfrsf25 Standard 2 vials, 10 ng/tube
EK1515-DA Mouse Tnfrsf25 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0410.0920.1220.1950.4010.8471.7412.402

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect DR3/TNFRSF25, Dilution ratio of 1:1, concentration in serum and plasma is around 1000 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNFRSF25/DR3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2061289433220112933972
Standard deviation8.4468.31316.2311.6568.52317.76
CV (%)4.1%5.3%7.3%5.8%5.3%8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of DR3/TNFRSF25 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120620922218920611.755.7%
Sample 21289116312091152120353.924.4%
Sample 343324412405446544363214.194.9%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf25 (Source: Uniprot.Org, NCBI)

Gene Name

Tnfrsf25

Full Name

Tumor necrosis factor receptor superfamily member 25

Weight

44264 MW

Alternative Names

Protein Tnfrsf25 ;Tumor necrosis factor receptor superfamily, member 25, isoform CRA_b ;Tnfrsf25 ;mCG_4090 ; TNFRSF25 APO-3, DDR3, DR3, GEF720, LARD, PLEKHG5, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR TNF receptor superfamily member 25 tumor necrosis factor receptor superfamily member 25|Guanine nucleotide exchange factor 720|PH domain-containing family G member 5|Pleckstrin homology domain-containing family G member 5|apoptosis inducing receptor|apoptosis-inducing receptor AIR|apoptosis-mediating receptor DR3|apoptosis-mediating receptor TRAMP|death domain receptor 3 soluble form|death receptor beta|lymphocyte-associated receptor of death|protein WSL-1|tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf25, check out the Tnfrsf25 Infographic

Tnfrsf25 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf25: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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7 Customer Q&As for Mouse TNFRSF25/DR3 ELISA Kit PicoKine®

Question

Q: how to proceed with the analysis of ELISA data? I have performed DR3 level in plasma.

M. Li

Verified customer

Asked: 2020-06-18

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-06-18

Question

Q: if the enzyme conjugated DR3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DR3 antigen?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-01-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

L. Smith

Verified customer

Asked: 2019-09-30

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-09-30

Question

Q: how can I thaw whole blood sample for DR3 ELISA after freezing?

R. Huang

Verified customer

Asked: 2019-05-11

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test DR3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-05-11

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

C. Jones

Verified customer

Asked: 2018-07-30

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-07-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

C. Edwards

Verified customer

Asked: 2018-04-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-04-05

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring DR3 using the EK1515 Mouse DR3 Picokine® ELISA Kit?

N. Gonzalez

Verified customer

Asked: 2016-08-22

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2016-08-22

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