Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit

Product Name

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit

See all Angiopoietin-like Protein 4/ANGPTL4 products

SKU/Catalog Number

FEK0960

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0960)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: L165-S406

Sensitivity

<300 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0960 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0960 is reactive to ANGPTL4 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0960 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0960-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0960-ST	Human Angiopoietin-like Protein 4/ANGPTL4 Standard	2	40 ng/tube
FEK0960-A	Human ANGPTL4 Antibody A	1	3ml
FEK0960-B	Human ANGPTL4 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.013	0.159	0.252	0.395	0.620	0.991	1.502	2.064

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 4/ANGPTL4 in ELISA kits from four different production batches/lots.

Gene/Protein Information For ANGPTL4 (Source: Uniprot.Org, NCBI)

Alternative Names

Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein ANGPTL4 ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158 angiopoietin like 4 angiopoietin-related protein 4|PPARG angiopoietin related protein|fasting-induced adipose factor|hepatic angiopoietin-related protein|hepatic fibrinogen/angiopoietin-related protein|peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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