Key=store_id
Key=entity_id, value=8975
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Key=type_id, value=simple
Key=sku, value=EK1579
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Key=created_at, value=2016-11-08 06:37:44
Key=updated_at, value=2024-09-26 00:48:18
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Key=status, value=Enabled
Key=name, value=Human TNFRSF25/DR3 ELISA Kit PicoKine®
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Key=custom_product_page, value=No
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Key=template, value=elisa-kits
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Key=googleshopping_exclude, value=No
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Key=yoast_content_score, value=60
Key=price, value=750.0000
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Key=meta_title, value=Human TNFRSF25/DR3 ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Human TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml.
Key=image, value=/e/k/ek1579_2.png
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Key=thumbnail, value=/e/k/ek1579_2.png
Key=url_key, value=human-tnfrsf25-dr3-picokine-trade-elisa-kit-ek1579-boster
Key=url_path, value=human-tnfrsf25-dr3-picokine-trade-elisa-kit-ek1579-boster
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Key=image_label, value=Human TNFRSF25/DR3 PicoKine ELISA Kit standard curve
Key=small_image_label, value=Human TNFRSF25/DR3 PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Human TNFRSF25/DR3 PicoKine ELISA Kit standard curve
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Key=size, value=96 wells/kit, with removable strips.
Key=protein_name, value=Tumor necrosis factor receptor superfamily member 25
Key=gene_full_name, value=Tumor necrosis factor receptor superfamily member 25
Key=uniprot_id, value=Q93038
Key=assay_range, value=312 pg/ml - 20,000 pg/ml
Key=sensitivity, value=<10 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=monoclonal antibody from mouse|polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=20,000 pg/ml, 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=predicted_reactivity
Key=source_company, value=Boster EK1579
Key=molecular_weight, value=45385 MW
Key=related_product_skus, value=A03227,EK1515,PA2004
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Apoptosis, Cell Biology, NFKB Pathway, Nuclear Signaling, Receptors, Signal Transduction, Signaling Pathway
Key=swatch_image, value=/e/k/ek1579_2.png
Key=rating_value, value=97.6
Key=review_count, value=5
Key=phospho_site
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Key=cj_related_products, value=T Cell Costimulation:EK0769|FEK0768|A03125-3|EK1730|EK1429|PA2136-2
Key=description, value=Human TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Human TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Human TNFRSF25/DR3 ELISA Kit, TNFRSF25/DR3 ELISA, Bosterbio High Sensitivity
Key=background, value=Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.
Key=gene_name, value=TNFRSF25
Key=synonyms, value=Tumor necrosis factor receptor superfamily member 25;Apo-3;Apoptosis-inducing receptor AIR;Apoptosis-mediating receptor DR3;Apoptosis-mediating receptor TRAMP;Death receptor 3;Lymphocyte-associated receptor of death;LARD;Protein WSL;Protein WSL-1;TNFRSF25;APO3, DDR3, DR3, TNFRSF12, WSL, WSL1;UNQ455/PRO779;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=20 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	691	2487	9783	668	2430	10603
Standard deviation	37.31	154.19	489.15	42.75	196.83	689.19
CV (%)	5.4%	6.2%	5%	6.4%	8.1%	6.5%

Key=application_details, value=Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 312 pg/ml.
Key=subcellular_localization, value= Isoform 1: Cell membrane; Single-pass type I membrane protein.
Key=tissue_specificity, value= Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.
Key=protein_function, value= Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.
Key=sample_data, value=

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.047	0.117	0.148	0.336	0.429	0.724	1.255	2.087

Key=competitor_equivalent_skus, value=
Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: Q25-F201
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	691	639	639	669	659	21.92	3.3%
Sample 2	2487	2310	2253	2294	2336	89.62	3.8%
Sample 3	9783	10046	9881	10103	9953	127.7	1.2%

*number of samples for each test n=16.
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