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Product Info Summary
| SKU: | EK1556 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Syndecan-3/SDC3 ELISA Kit PicoKine®
SKU/Catalog Number
EK1556
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Syndecan-3/SDC3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Sdc3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Syndecan-3/SDC3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1556)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A45-V387
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1556 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1556 is reactive to Sdc3 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1556 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SDC3
Syndecan-3 is a protein that in humans is encoded by the SDC3 gene. The protein encoded by this gene belongs to the syndecan proteoglycan family, which was assigned to human chromosome 1p35.2, and it showed that in the mouse the Synd3 gene maps to chromosome 4 near the Lmyc gene. The transfection of SDC3 resulted in the formation of long filopodia-like structures, microspikes, and varicosities in several cell lines. Besides, the gene plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1556-CAP | Anti-Mouse Sdc3 Pre-coated 96-well strip microplate | 1 |
| EK1556-ST | Mouse Sdc3 Standard | 2 vials, 10 ng/tube |
| EK1556-DA | Mouse Sdc3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.077 | 0.161 | 0.212 | 0.322 | 0.550 | 1.064 | 1.694 | 2.086 |
Data Example Images
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Mouse Syndecan-3/SDC3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SDC3, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 156 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Syndecan-3/SDC3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 2280 | 1800 | 4508 | 280 | 1660 | 4073 |
| Standard deviation | 17.8%3 | 140.4 | 193.84 | 20.16 | 129.48 | 236.23 |
| CV (%) | 5.3% | 7.8% | 4.3% | 7.2% | 7.8% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SDC3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 295 | 333 | 330 | 288 | 311 | 20.18 | 6.4% |
| Sample 2 | 1800 | 1725 | 1781 | 1910 | 1804 | 67.12 | 3.7% |
| Sample 3 | 4508 | 4397 | 4649 | 3887 | 4360 | 287.45 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For Sdc3 (Source: Uniprot.Org, NCBI)
Gene Name
Sdc3
Full Name
Syndecan-3
Weight
46002 MW
Superfamily
syndecan proteoglycan family
Alternative Names
Syndecan-3;SYND3;Sdc3;Kiaa0468; SDC3 SDCN, SYND3 syndecan 3 syndecan-3|N-syndecan|syndecan neural type|syndecan proteoglycan 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Sdc3, check out the Sdc3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Sdc3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Syndecan-3/SDC3 ELISA Kit PicoKine® (EK1556)
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No publications found for EK1556
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse Syndecan-3/SDC3 ELISA Kit PicoKine®
Question
Q: Can SDC3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Maharaj
Verified customer
Asked: 2020-07-06
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-07-06
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of SDC3 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
P. Davis
Verified customer
Asked: 2019-10-13
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SDC3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-10-13
Question
Q: is it okay to use citrate plasma as samples in Mouse SDC3 Picokine® ELISA Kit (Catalog # EK1556)?
M. Johnson
Verified customer
Asked: 2018-12-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of SDC3 causing degradation of its protein structure. SDC3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the SDC3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-12-25
Question
Q: how to proceed with the analysis of ELISA data? I have performed SDC3 level in plasma.
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-09-27
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
P. Puri
Verified customer
Asked: 2018-06-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-06-12
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
M. Green
Verified customer
Asked: 2015-04-15
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-04-15
