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Product Info Summary
| SKU: | EK2109 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human CANT1 ELISA Kit PicoKine®
View all Calcium Activated Nucleotidase 1/CANT1 ELISA kits
SKU/Catalog Number
EK2109
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CANT1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CANT1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CANT1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2109)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: R63-I401
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2109 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2109 is reactive to CANT1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2109 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Calcium Activated Nucleotidase 1/CANT1
CANT1 is a gene that encodes soluble calcium-activated nucleotidase 1, an enzyme, in humans. It is mapped to 17q25.3. This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2109-CAP | Anti-Human CANT1 Pre-coated 96-well strip microplate | 1 |
| EK2109-ST | Human CANT1 Standard | 2 vials, 10 ng/tube |
| EK2109-DA | Human CANT1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.019 | 0.065 | 0.152 | 0.286 | 0.494 | 0.865 | 1.493 | 2.000 |
Data Example Images
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Human CANT1 PicoKine ELISA Kit
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Calcium Activated Nucleotidase 1/CANT1, Dilution ratio of 1:1, concentration in serum and plasma is around 4 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CANT1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 301 | 1170 | 4798 | 307 | 1195 | 4812 |
| Standard deviation | 15.65 | 62.01 | 259.09 | 18.73 | 70.51 | 327.22 |
| CV (%) | 5.2% | 5.3% | 5.4% | 6.1% | 5.9% | 6.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Calcium Activated Nucleotidase 1/CANT1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 319 | 341 | 313 | 295 | 317 | 16.43 | 5.1% |
| Sample 2 | 1085 | 1189 | 1030 | 1018 | 1080 | 67.54 | 6.2% |
| Sample 3 | 5570 | 5244 | 5973 | 5762 | 5637 | 268.07 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For CANT1 (Source: Uniprot.Org, NCBI)
Gene Name
CANT1
Full Name
Soluble calcium-activated nucleotidase 1
Weight
45.653kDa
Superfamily
apyrase family
Alternative Names
Soluble calcium-activated nucleotidase 1; SCAN-1; Apyrase homolog; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107; CANT1; SHAPY Cant1|5830420C20Rik, Apy, Apy1h, D11Bwg0554e, Entpd8, SCA, SCAN-1, Sh, Shapy|calcium activated nucleotidase 1|soluble calcium-activated nucleotidase 1|Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase|apyrase 1, homolog (C. lectularius)|apyrase homolog
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CANT1, check out the CANT1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CANT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CANT1 ELISA Kit PicoKine® (EK2109)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Human CANT1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Green
Verified customer
Asked: 2019-11-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-11-18
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CANT1 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-05-11
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CANT1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-05-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CANT1 using the EK2109 Human CANT1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-08-22
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
J. Miller
Verified customer
Asked: 2016-02-07
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-02-07
