Human CANT1 ELISA Kit PicoKine®

Calcium Activated Nucleotidase 1/CANT1 ELISA kit for Human

Human CANT1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CANT1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2109
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human CANT1 ELISA Kit PicoKine®

View all Calcium Activated Nucleotidase 1/CANT1 ELISA kits

SKU/Catalog Number

EK2109

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CANT1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CANT1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CANT1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2109)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R63-I401

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2109 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2109 is reactive to Cant1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2109 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Calcium Activated Nucleotidase 1/CANT1

CANT1 is a gene that encodes soluble calcium-activated nucleotidase 1, an enzyme, in humans. It is mapped to 17q25.3. This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene.

Kit Components

Catalog Number Description Quantity
EK2109-CAP Anti-Human Cant1 Pre-coated 96-well strip microplate 1
EK2109-ST Human Cant1 Standard 2 vials, 10 ng/tube
EK2109-DA Human Cant1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)01563126251,2502,5005,00010,000
O.D.0.0190.0650.1520.2860.4940.8651.4932.000

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Calcium Activated Nucleotidase 1/CANT1, Dilution ratio of 1:1, concentration in serum and plasma is around 4 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CANT1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3011170479830711954812
Standard deviation15.6562.01259.0918.7370.51327.22
CV (%)5.2%5.3%5.4%6.1%5.9%6.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Calcium Activated Nucleotidase 1/CANT1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131934131329531716.435.1%
Sample 21085118910301018108067.546.2%
Sample 355705244597357625637268.074.7%
*number of samples for each test n=16.

Gene/Protein Information For Cant1 (Source: Uniprot.Org, NCBI)

Gene Name

Cant1

Full Name

Soluble calcium-activated nucleotidase 1

Weight

45.653kDa

Superfamily

apyrase family

Alternative Names

Apyrase homolog; calcium activated nucleotidase 1; CANT1; DBQD; EC 3.6.1.6; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107; SCAN1; SCAN-1Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase; SHAPY; SHAPYmicromelic dwarfism with vertebral and metaphyseal abnormalities and advancedcarpotarsal ossification; soluble Ca-activated nucleotidase, isozyme 1; soluble calcium-activated nucleotidase 1; soluble calcium-activated nucleotidase SCAN-1 Cant1|5830420C20Rik, Apy, Apy1h, D11Bwg0554e, Entpd8, SCA, SCAN-1, Sh, Shapy|calcium activated nucleotidase 1|soluble calcium-activated nucleotidase 1|Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase|apyrase 1, homolog (C. lectularius)|apyrase homolog

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cant1, check out the Cant1 Infographic

Cant1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cant1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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4 Customer Q&As for Human CANT1 ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

M. Green

Verified customer

Asked: 2019-11-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-11-18

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CANT1 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-05-11

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CANT1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-05-11

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CANT1 using the EK2109 Human CANT1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-08-22

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-08-22

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

J. Miller

Verified customer

Asked: 2016-02-07

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-02-07

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