Click image to see more details
Product Info Summary
| SKU: | EK1989 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin) |
Product info
Product Name
Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine®
View all Stanniocalcin 2/STC-2 ELISA kits
SKU/Catalog Number
EK1989
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse STC2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse STC2 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1989)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T25-R296
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1989 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1989 is reactive to Stc2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin)
Application Guarantee
EK1989 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Stanniocalcin 2/STC-2
Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 11; 11 A4. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1989-CAP | Anti-Mouse Stc2 Pre-coated 96-well strip microplate | 1 |
| EK1989-ST | Mouse Stc2 Standard | 2 vials, 10 ng/tube |
| EK1989-DA | Mouse Stc2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.004 | 0.065 | 0.105 | 0.237 | 0.472 | 0.729 | 1.009 | 1.852 |
Data Example Images
Click image to see more details
Mouse STC2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Stanniocalcin 2/STC-2, Dilution ratio of 1:8, concentration in serum and plasma is around 15 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 247 | 1255 | 4504 | 284 | 1264 | 4728 |
| Standard deviation | 12.84 | 56.48 | 256.73 | 19.60 | 73.31 | 297.86 |
| CV (%) | 5.2% | 4.5% | 5.7% | 6.9% | 5.8% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Stanniocalcin 2/STC-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 247 | 253 | 236 | 226 | 240 | 10.35 | 4.3% |
| Sample 2 | 1255 | 1282 | 1191 | 1248 | 1244 | 33.12 | 2.6% |
| Sample 3 | 4504 | 4761 | 4782 | 5438 | 4871 | 345.03 | 7.0% |
Protein Target Info & Infographic
Gene/Protein Information For Stc2 (Source: Uniprot.Org, NCBI)
Gene Name
Stc2
Full Name
Stanniocalcin-2
Weight
33.249kDa
Superfamily
stanniocalcin family
Alternative Names
Stanniocalcin-2; STC-2; Stc2 STC2 STC-2, STCRP stanniocalcin 2 stanniocalcin-2|STC-related protein|stanniocalcin-related protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Stc2, check out the Stc2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Stc2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine® (EK1989)
Hello CJ!
No publications found for EK1989
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-12-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-12-18
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Stanniocalcin 2 using the EK1989 Mouse Stanniocalcin 2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-11-19
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-11-19
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-10-17
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-10-17
Question
Q: What is the optimal O.D. value for Stanniocalcin 2 ELISA kit? I performed your Stanniocalcin 2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Stanniocalcin 2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-09-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Stanniocalcin 2 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Stanniocalcin 2 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-09-18
Question
Q: can you suggest the dilution ratio of serum samples for detection of Stanniocalcin 2 in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-06-08
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Stanniocalcin 2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-06-08
Question
Q: how to analyze ELISA data? I have performed Stanniocalcin 2 level in serum.
S. Davis
Verified customer
Asked: 2019-03-06
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-03-06
Question
Q: how can I thaw whole blood sample for Stanniocalcin 2 ELISA after freezing?
A. Hernandez
Verified customer
Asked: 2018-05-23
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Stanniocalcin 2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-05-23
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
B. Dhawan
Verified customer
Asked: 2016-10-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-10-06
Question
Q: if the enzyme conjugated Stanniocalcin 2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Stanniocalcin 2 antigen?
Verified Customer
Verified customer
Asked: 2016-01-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-01-06
