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Product Info Summary
| SKU: | EK1988 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum |
Product info
Product Name
Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine®
View all Stanniocalcin 2/STC-2 ELISA kits
SKU/Catalog Number
EK1988
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine® (96 Tests). Quantitate Human STC2 in cell culture supernatants and serum. Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1988)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T25-R302
Sensitivity
<5 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of EK1988 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1988 is reactive to STC2 in Human samples
Validated Sample Types
cell culture supernatants and serum
Application Guarantee
EK1988 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Stanniocalcin 2/STC-2
Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 5q35.2. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1988-CAP | Anti-Human STC2 Pre-coated 96-well strip microplate | 1 |
| EK1988-ST | Human STC2 Standard | 2 vials, 10 ng/tube |
| EK1988-DA | Human STC2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.033 | 0.082 | 0.135 | 0.214 | 0.402 | 0.731 | 1.288 | 2.062 |
Data Example Images
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Human STC2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Stanniocalcin 2/STC-2, Dilution ratio of 1:1, concentration in serum is 15-70 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 9 | 71 | 207 | 12 | 75 | 219 |
| Standard deviation | 0.38 | 3.62 | 11.39 | 0.82 | 4.43 | 13.36 |
| CV (%) | 4.2% | 5.1% | 5.5% | 6.8% | 5.9% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Stanniocalcin 2/STC-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 9 | 10 | 9 | 10 | 9 | 0.50 | 5.5% |
| Sample 2 | 71 | 72 | 61 | 64 | 67 | 4.63 | 6.9% |
| Sample 3 | 207 | 217 | 226 | 208 | 214 | 7.69 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For STC2 (Source: Uniprot.Org, NCBI)
Gene Name
STC2
Full Name
Stanniocalcin-2
Weight
33.249kDa
Superfamily
stanniocalcin family
Alternative Names
Stanniocalcin-2; STC-2; Stanniocalcin-related protein; STC-related protein; STCRP; STC2 STC2 STC-2, STCRP stanniocalcin 2 stanniocalcin-2|STC-related protein|stanniocalcin-related protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on STC2, check out the STC2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for STC2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine® (EK1988)
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No publications found for EK1988
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I have performed Stanniocalcin 2 level in serum.
D. Sunder
Verified customer
Asked: 2020-02-04
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-04
Question
Q: Can Stanniocalcin 2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-10-19
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-10-19
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Stanniocalcin 2 using the EK1988 Human Stanniocalcin 2 Picokine® ELISA Kit?
W. Huang
Verified customer
Asked: 2019-07-28
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-07-28
Question
Q: if the enzyme conjugated Stanniocalcin 2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Stanniocalcin 2 antigen?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-01-22
Question
Q: can you recommend the dilution ratio of serum samples for detection of Stanniocalcin 2 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Stanniocalcin 2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-10-11
Question
Q: how to thaw whole blood sample for Stanniocalcin 2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-05-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Stanniocalcin 2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-05-02
