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Product Info Summary
| SKU: | A01313 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Heparanase 1/Hpse Antibody Picoband®
View all Heparanase/HPSE Antibodies
SKU/Catalog Number
A01313
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Heparanase 1/Hpse Antibody Picoband® catalog # A01313. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heparanase 1/Hpse Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01313)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Heparanase 1 recombinant protein (Position: K206-K457). Mouse Heparanase 1 shares 80.2% and 96.4% amino acid (aa) sequence identity with human and rat Heparanase 1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01313 is reactive to Hpse in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
60066 MW
Background of Heparanase/HPSE
Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01313 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody (A01313).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody (Catalog # A01313) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 65KD. The expected band size for Heparanase 1 is at 61KD.
Protein Target Info & Infographic
Gene/Protein Information For Hpse (Source: Uniprot.org, NCBI)
Gene Name
Hpse
Full Name
Heparanase
Weight
60066 MW
Superfamily
glycosyl hydrolase 79 family
Alternative Names
Heparanase;3.2.1.166;Endo-glucoronidase;Heparanase 8 kDa subunit;Heparanase 50 kDa subunit;Hpse;Hpa; HPSE HPA, HPA1, HPR11, HSE1, HPSE heparanase heparanase|endo-glucoronidase|heparanase-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Hpse, check out the Hpse Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hpse: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Heparanase 1/Hpse Antibody Picoband® (A01313)
Hello CJ!
A01313 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of choriocarcinoma by Fe3O4-dextran-anti-?-human chorionic gonadotropin nanoparticles containing antisense oligodeoxynucleotide of heparanase
Qin Q, Xu G, Qi W, Guo M, Wang Z, Xu W, Qiao Z, Gu Y, Niu J. Exp Ther Med. 2017 Aug;14(2):1017-1022. doi: 10.3892/etm.2017.4609. Epub 2017 Jun 15. Evaluation of the C-domain of heparanase during AGE-induced macrophage inflammatory response
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