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Product Info Summary
| SKU: | EK1969 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA, citrate) and urine. |
Product info
Product Name
Mouse Serpin D1 ELISA Kit PicoKine®
View all Serpin D1/Heparin Cofactor II ELISA kits
SKU/Catalog Number
EK1969
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Serpin D1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Serpind1 in cell culture supernatants, serum and plasma (EDTA, citrate) and urine. Sensitivity: 0.1ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Serpin D1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1969)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E24-S478
Sensitivity
<0.1 ng/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK1969 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1969 is reactive to SERPIND1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA, citrate) and urine.
Application Guarantee
EK1969 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin D1/Heparin Cofactor II
Heparin cofactor II (HCII), a protein encoded by the SERPIND1 gene, is a coagulation factor that inhibits IIa, and is a cofactor for heparin and dermatan sulfate. It is mapped to 16 A3; 16 10.74 cM. This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1969-CAP | Anti-Mouse SERPIND1 Pre-coated 96-well strip microplate | 1 |
| EK1969-ST | Mouse SERPIND1 Standard | 2 vials, 50 ng/tube |
| EK1969-DA | Mouse SERPIND1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.033 | 0.328 | 0.448 | 0.857 | 1.077 | 1.482 | 1.954 | 2.310 |
Data Example Images
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Mouse Serpin D1 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin D1/Heparin Cofactor II, Dilution ratio of 1:500, concentration in serum and plasma is around 4.2 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Serpin D1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1219 | 5460 | 26552 | 1229 | 6087 | 28436 |
| Standard deviation | 54.86 | 354.90 | 1778.98 | 83.57 | 359.13 | 1734.60 |
| CV (%) | 4.5% | 6.5% | 6.7% | 6.8% | 5.9% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin D1/Heparin Cofactor II in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1219 | 1130 | 1188 | 1149 | 1171 | 34.48 | 2.9% |
| Sample 2 | 5460 | 5176 | 5677 | 5786 | 5524 | 233.05 | 4.2% |
| Sample 3 | 26552 | 26436 | 26634 | 25685 | 26326 | 377.13 | 1.4% |
Protein Target Info & Infographic
Gene/Protein Information For SERPIND1 (Source: Uniprot.Org, NCBI)
Gene Name
SERPIND1
Full Name
Heparin cofactor 2
Weight
Superfamily
serpin family
Alternative Names
HC2; HC2LS2; HCF2; HCF2clade D (heparin cofactor), member 1; HC-II; Heparin Cofactor II; HLS2HCII; leuserpin 2; LS2; Protease inhibitor leuserpin-2; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1 SERPIND1 D22S673, HC2, HCF2, HCII, HLS2, LS2, THPH10 serpin family D member 1 heparin cofactor 2|heparin cofactor II splice isoform|leuserpin 2|protease inhibitor leuserpin-2|serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1|serpin D1|serpin peptidase inhibitor, clade D (heparin cofactor), member 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SERPIND1, check out the SERPIND1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SERPIND1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Serpin D1 ELISA Kit PicoKine® (EK1969)
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Customer Q&As
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8 Customer Q&As for Mouse Serpin D1 ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I have performed Serpin D1 level in serum.
Verified Customer
Verified customer
Asked: 2020-08-07
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-08-07
Question
Q: can I use heparin plasma as samples in Mouse Serpin D1 Picokine® ELISA Kit (Catalog # EK1969)?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Serpin D1 causing degradation of its protein structure. Serpin D1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Serpin D1 ELISA, treating samples with a number of anticoagulants and decided that EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-30
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Serpin D1 using the EK1969 Mouse Serpin D1 Picokine® ELISA Kit?
W. Hall
Verified customer
Asked: 2019-01-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-01-11
Question
Q: What is the optimal O.D. value for Serpin D1 ELISA kit? I used your Serpin D1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Serpin D1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin D1 ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of Serpin D1 that can be declared/interpreted as positive by the above standard is 0.1ng/ml.
Boster Scientific Support
Answered: 2018-03-01
Question
Q: can you recommend the dilution ratio of serum samples for detection of Serpin D1 in Mouse serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Serpin D1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-10-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Walker
Verified customer
Asked: 2017-06-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-06-10
Question
Q: Can Serpin D1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2017-02-20
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-02-20
Question
Q: how to thaw whole blood sample for Serpin D1 ELISA after freezing?
S. Carter
Verified customer
Asked: 2016-07-30
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin D1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-07-30
