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Product Info Summary
| SKU: | EK0723 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Mouse CXCL1/Gro Alpha ELISA Kit PicoKine®
View all CXCL1/GRO alpha/KC/CINC-1 ELISA kits
SKU/Catalog Number
EK0723
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CXCL1/Gro Alpha ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cxcl1 in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CXCL1/Gro Alpha ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0723)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: R20-K96
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0723 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0723 is reactive to Cxcl1 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0723 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL1/GRO alpha/KC/CINC-1
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0723-CAP | Anti-Mouse Cxcl1 Pre-coated 96-well strip microplate | 1 |
| EK0723-ST | Mouse Cxcl1 Standard | 2 vials, 10 ng/tube |
| EK0723-DA | Mouse Cxcl1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.080 | 0.130 | 0.171 | 0.302 | 0.579 | 0.984 | 1.660 | 2.065 |
Data Example Images
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Mouse CXCL1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL1/GRO alpha/KC/CINC-1, Dilution ratio of 1:1, concentration in serum is 32-306 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CXCL1/Gro Alpha ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 28 | 242 | 501 | 27 | 253 | 536 |
| Standard deviation | 1.42 | 16.45 | 37.07 | 1.67 | 21.25 | 45.02 |
| CV (%) | 8.4%% | 6.8% | 7.4% | 6.2% | 8.4% | 8.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL1/GRO alpha/KC/CINC-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 28 | 29 | 31 | 27 | 28 | 1.47 | 5.2% |
| Sample 2 | 242 | 253 | 228 | 272 | 248 | 16.08 | 6.4% |
| Sample 3 | 501 | 480 | 502 | 525 | 502 | 15.92 | 3.1% |
Protein Target Info & Infographic
Gene/Protein Information For Cxcl1 (Source: Uniprot.Org, NCBI)
Gene Name
Cxcl1
Full Name
Growth-regulated alpha protein
Weight
10254 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine ;Cxcl1 ;mCG_1708 ; CXCL1 FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1 C-X-C motif chemokine ligand 1 growth-regulated alpha protein|C-X-C motif chemokine 1|GRO-alpha(1-73)|GRO1 oncogene (melanoma growth stimulating activity, alpha)|GRO1 oncogene (melanoma growth-stimulating activity)|MGSA alpha|chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)|fibroblast secretory protein|melanoma growth stimulating activity, alpha|melanoma growth stimulatory activity alpha|neutrophil-activating protein 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cxcl1, check out the Cxcl1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cxcl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CXCL1/Gro Alpha ELISA Kit PicoKine® (EK0723)
Hello CJ!
EK0723 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Astragaloside IV Promotes Adult Neurogenesis in Hippocampal Dentate Gyrus of Mouse through CXCL1/CXCR2 Signaling
Comparative mouse lung injury by nickel nanoparticles with differential surface modification
Tumor cells educate mesenchymal stromal cells to release chemoprotective and immunomodulatory factors
miR-21 mediates nickel nanoparticle-induced pulmonary injury and fibrosis
Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma
Photodynamic/ photothermal therapy enhances neutrophil-mediated ibrutinib tumor delivery for potent tumor immunotherapy: More than one plus one?
Preclinical development of GR1501, a human monoclonal antibody that neutralizes interleukin-17A
Cross‐Strand Interaction, Central Bending, and Sequence Pattern Act as Biomodulators of Simplified β‐Hairpin Antimicrobial Amphiphiles
Species: Mouse
Wan R, Mo Y, Zhang Z, Jiang M, Tang S, Zhang Q. Part Fibre Toxicol. 2017 Sep 18;14(1):38. doi: 10.1186/s12989-017-0219-z. Cobalt nanoparticles induce lung injury, DNA damage and mutations in mice
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse CXCL1/Gro Alpha ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-06-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-06-20
Question
Has the CXCL1 Elisa kit been tested for CXCL2 cross reactivity?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
No. We have not tested this kit for CXCL2.
Boster Scientific Support
Answered: 2019-11-29
Question
Q: which procedure should I follow in order to thaw whole blood sample for CXCL1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-08-19
Question
Q: can you recommend the dilution ratio of serum samples for detection of CXCL1 in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-03-04
Question
Q: Can CXCL1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Anderson
Verified customer
Asked: 2019-02-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-02-16
Question
Has this kit been validated to work on serum? Is there serum data available?
Verified Customer
Verified customer
Asked: 2018-07-04
Answer
This kit can work on serum. The detection range is about 38 to 279 pg/mL.
Boster Scientific Support
Answered: 2018-07-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CXCL1 using the EK0723 Mouse CXCL1 Picokine® ELISA Kit?
S. Krishna
Verified customer
Asked: 2018-05-19
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-05-19
Question
Q: if the enzyme conjugated CXCL1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL1 antigen?
M. Wilson
Verified customer
Asked: 2018-03-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-03-10
Question
Q: how to analyze ELISA data? I have performed CXCL1 level in serum.
Verified Customer
Verified customer
Asked: 2015-12-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-12-07
