Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody

Product Name

Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody

View all S100A8 & S100A9 Antibodies

SKU/Catalog Number

M00413

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody (Catalog # M00413). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Dog, Rabbit, Guinea pig, Rat, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00413)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM281

Isotype

IgG1, kappa

Immunogen

Affinity Purified monocyte membrane preparation

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00413 is reactive to S100A8 & S100A9 in Cow, Dog, Goat, Guinea pig, Human, Monkey, Mouse, Pig, Rabbit, Rat, Horse, Cat, Baboon

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Calculated molecular weight

10835 MW

Background of S100A8 & S100A9

Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This monoclonal antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00413 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For S100A8 & S100A9 (Source: Uniprot.org, NCBI)

Alternative Names

Protein S100-A8;Calgranulin-A;Calprotectin L1L subunit;Cystic fibrosis antigen;CFAG;Leukocyte L1 complex light chain;Migration inhibitory factor-related protein 8;MRP-8;p8;S100 calcium-binding protein A8;Urinary stone protein band A;Protein S100-A8, N-terminally processed;S100A8;CAGA, CFAG, MRP8;

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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