Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody

Boster Bio Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody (Catalog # M00413). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Dog, Rabbit, Guinea pig, Rat, Mouse.

Product Info Summary

SKU: M00413
Size: 100ug/vial
Reactive Species: Cow, Dog, Goat, Guinea pig, Human, Monkey, Mouse, Pig, Rabbit, Rat, Horse, Cat, Baboon
Host: Mouse
Application: Flow Cytometry, IF, IHC

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Product Name

Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody

View all Antibodies

SKU/Catalog Number

M00413

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody (Catalog # M00413). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Dog, Rabbit, Guinea pig, Rat, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00413)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM281

Isotype

IgG1, kappa

Immunogen

Affinity Purified monocyte membrane preparation

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00413 is reactive to in Cow, Dog, Goat, Guinea pig, Human, Monkey, Mouse, Pig, Rabbit, Rat, Horse, Cat, Baboon

Applications

M00413 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

10835 MW

Background of

Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This monoclonal antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with 1ml water (15 minutes at room temperature).

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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3 Customer Q&As for Anti-Macrophage L1 Protein S100A8 Monoclonal Antibody

Question

We were content with the WB result of your anti-Macrophage L1 Protein Monoclonal antibody. However we have seen positive staining in neutrophil secreted. cytoplasm. cytoplasm, using this antibody. Is that expected? Could you tell me where is S100A8 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

According to literature, neutrophil does express S100A8. Generally S100A8 expresses in secreted. cytoplasm. cytoplasm,. Regarding which tissues have S100A8 expression, here are a few articles citing expression in various tissues:
Ascites, Pubmed ID: 7695842
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 24275569
Neutrophil, Pubmed ID: 1326551
Skeletal muscle, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-07-30

Question

We have seen staining in monkey keratinocyte. What should we do? Is anti-Macrophage L1 Protein Monoclonal antibody supposed to stain keratinocyte positively?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

According to literature keratinocyte does express S100A8. According to Uniprot.org, S100A8 is expressed in pharyngeal mucosa, tongue, skeletal muscle, neutrophil, ascites, keratinocyte, liver, among other tissues. Regarding which tissues have S100A8 expression, here are a few articles citing expression in various tissues:
Ascites, Pubmed ID: 7695842
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 24275569
Neutrophil, Pubmed ID: 1326551
Skeletal muscle, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-06-07

Question

We are currently using anti-Macrophage L1 Protein Monoclonal antibody M00413 for goat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says bovine, canine, equine, goat, guinea pig, human, monkey, pig, rabbit, rat. Is it possible that the antibody can work on feline tissues as well?

K. Singh

Verified customer

Asked: 2014-04-08

Answer

The anti-Macrophage L1 Protein Monoclonal antibody (M00413) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-04-08

Order DetailsPrice
M00413-100ug

100 μg

$468

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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