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Product Info Summary
| SKU: | EK0338 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse FASL ELISA Kit PicoKine®
View all Fas Ligand/TNFSF6 ELISA kits
SKU/Catalog Number
EK0338
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse FASL ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Faslg in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse FASL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0338)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: P132-L279
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0338 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0338 is reactive to Faslg in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0338 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Fas Ligand/TNFSF6
FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0338-CAP | Anti-Mouse Faslg Pre-coated 96-well strip microplate | 1 |
| EK0338-ST | Mouse Faslg Standard | 2 vials, 10 ng/tube |
| EK0338-DA | Mouse Faslg Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.016 | 0.074 | 0.127 | 0.232 | 0.456 | 0.863 | 1.521 | 2.210 |
Data Example Images
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Mouse FASL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Fas Ligand/TNFSF6, Dilution ratio of 1:1, concentration in serum and plasma is 0-165 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse FASL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 68 | 206 | 772 | 65 | 216 | 727 |
| Standard deviation | 2.99 | 13.59 | 49.4 | 3.51 | 17.28 | 47.98 |
| CV (%) | 4.4% | 6.6% | 6.4% | 5.4% | 8% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Fas Ligand/TNFSF6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 68 | 63 | 68 | 59 | 64 | 3.77 | 5.8% |
| Sample 2 | 206 | 196 | 205 | 225 | 208 | 10.55 | 5% |
| Sample 3 | 772 | 915 | 764 | 889 | 835 | 67.68 | 8.1% |
Protein Target Info & Infographic
Gene/Protein Information For Faslg (Source: Uniprot.Org, NCBI)
Gene Name
Faslg
Full Name
Tumor necrosis factor ligand superfamily member 6
Weight
31442 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 6;CD95 ligand;CD95-L;Fas antigen ligand;Fas ligand;FasL;CD178;Tumor necrosis factor ligand superfamily member 6, membrane form;Tumor necrosis factor ligand superfamily member 6, soluble form;Receptor-binding FasL ectodomain;Soluble Fas ligand;sFasL;ADAM10-processed FasL form;APL;FasL intracellular domain;FasL ICD;SPPL2A-processed FasL form;SPA;Faslg;Apt1lg1, Cd95l, Fasl, gld, Tnfsf6; FASLG ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A Fas ligand tumor necrosis factor ligand superfamily member 6|CD95 ligand|Fas ligand (TNF superfamily, member 6)|apoptosis (APO-1) ligand 1|apoptosis ligand|fas ligand|mutant tumor necrosis factor family member 6|tumor necrosis factor ligand 1A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Faslg, check out the Faslg Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Faslg: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse FASL ELISA Kit PicoKine® (EK0338)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse FASL ELISA Kit PicoKine®
Question
Q: can you suggest the dilution ratio of serum samples for detection of Fas Ligand in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
H. Baker
Verified customer
Asked: 2020-11-22
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Fas Ligand ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-22
Question
Q: Can Fas Ligand ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
P. Krishna
Verified customer
Asked: 2020-11-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-11-07
Question
Q: if the enzyme conjugated Fas Ligand antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Fas Ligand antigen?
C. Perez
Verified customer
Asked: 2020-06-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-06-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-27
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Fas Ligand using the EK0338 Mouse Fas Ligand Picokine® ELISA Kit?
L. Young
Verified customer
Asked: 2019-10-14
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-10-14
Question
Q: how to thaw whole blood sample for Fas Ligand ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Fas Ligand for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-11-28
