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Product Info Summary
| SKU: | PB9965 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-XIAP Antibody Picoband®
SKU/Catalog Number
PB9965
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-XIAP Antibody Picoband® catalog # PB9965. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XIAP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9965)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human XIAP recombinant protein (Position: A15-V244). Human XIAP shares 89.4% and 90.7% amino acid (aa) sequence identity with mouse and rat XIAP, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9965 is reactive to XIAP in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
56685 MW
Background of XIAP
XIAP, also known as IAP3 or BIRC4, is a protein that stops apoptotic cell death. It is mapped to chromosome Xq25. This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9965 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of XIAP using anti-XIAP antibody (PB9965).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XIAP antigen affinity purified polyclonal antibody (Catalog # PB9965) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XIAP at approximately 55 kDa. The expected band size for XIAP is at 57 kDa.
Protein Target Info & Infographic
Gene/Protein Information For XIAP (Source: Uniprot.org, NCBI)
Gene Name
XIAP
Full Name
E3 ubiquitin-protein ligase XIAP
Weight
56685 MW
Superfamily
IAP family
Alternative Names
E3 ubiquitin-protein ligase XIAP;6.3.2.-;Baculoviral IAP repeat-containing protein 4;IAP-like protein;ILP;hILP;Inhibitor of apoptosis protein 3;IAP-3;hIAP-3;hIAP3;X-linked inhibitor of apoptosis protein;X-linked IAP;XIAP;API3, BIRC4, IAP3; XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 X-linked inhibitor of apoptosis E3 ubiquitin-protein ligase XIAP|IAP-like protein 1|RING-type E3 ubiquitin transferase XIAP|X-linked IAP|X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase|baculoviral IAP repeat-containing protein 4|inhibitor of apoptosis protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XIAP, check out the XIAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XIAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XIAP Antibody Picoband® (PB9965)
Hello CJ!
PB9965 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wei H, Gao Z, Zheng L, Zhang C, Liu Z, Yang Y, Teng H, Hou L, Yin Y, Zou X0. Mar Drugs. 2017 Mar 16;15(3). pii: E77. doi: 10.3390/md15030077. Protective Effects of Fucoidan on Aβ25–35 and d-Gal-Induced Neurotoxicity in PC12 Cells and d-Gal-Induced...
Chen Gg, Mao M, Qiu Lz, Liu Qm. Neural Regen Res. 2015 Mar;10(3):425-31. Doi: 10.4103/1673-5374.153691. Gene Transfection Mediated By Polyethyleneimine-Polyethylene Glycol Nanocarrier Prevents Cisplatin-Induced Spiral Ganglion Cell Damage.
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Customer Q&As
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1 Customer Q&As for Anti-XIAP Antibody Picoband®
Question
We are currently using anti-XIAP antibody PB9965 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
G. Baker
Verified customer
Asked: 2019-03-05
Answer
The anti-XIAP antibody (PB9965) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-05
