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Product Info Summary
| SKU: | EK2050 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse CD206/CLEC13D ELISA Kit PicoKine®
View all MMR/CD206/Mannose Receptor ELISA kits
SKU/Catalog Number
EK2050
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CD206/CLEC13D ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mrc1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CD206/CLEC13D ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2050)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L20-A1388
Sensitivity
<50 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK2050 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2050 is reactive to Mrc1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2050 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMR/CD206/Mannose Receptor
The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). This gene is mapped to 2 A2; 2 10.46 cM. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2050-CAP | Anti-Mouse Mrc1 Pre-coated 96-well strip microplate | 1 |
| EK2050-ST | Mouse Mrc1 Standard | 2 vials, 50 ng/tube |
| EK2050-DA | Mouse Mrc1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.049 | 0.188 | 0.306 | 0.514 | 0.979 | 1.383 | 1.708 | 2.056 |
Data Example Images
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Mouse CD206/CLEC13D PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMR/CD206/Mannose Receptor, Dilution ratio of 1:10, concentration in serum and plasma is around 100 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD206/CLEC13D ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1150 | 6191 | 22279 | 1234 | 6199 | 23488 |
| Standard deviation | 59.80 | 266.21 | 913.44 | 81.44 | 359.54 | 1550.21 |
| CV (%) | 5.2% | 4.3% | 4.1% | 6.6% | 5.8% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMR/CD206/Mannose Receptor in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1150 | 1144 | 1176 | 1080 | 1137 | 35.30 | 3.1% |
| Sample 2 | 6191 | 5982 | 6880 | 6356 | 6352 | 332.27 | 5.2% |
| Sample 3 | 22279 | 22618 | 21731 | 19374 | 21500 | 1267.87 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For Mrc1 (Source: Uniprot.Org, NCBI)
Gene Name
Mrc1
Full Name
Macrophage mannose receptor 1
Weight
166.012kDa
Alternative Names
Macrophage mannose receptor 1; MMR; CD206; Mrc1 MRC1 CD206, CLEC13D, CLEC13DL, MMRL1, bA541I19.1, hMR, MRC1 mannose receptor C-type 1 macrophage mannose receptor 1|C-type lectin domain family 13 member D|human mannose receptor|macrophage mannose receptor 1-like protein 1|mannose receptor, C type 1-like 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Mrc1, check out the Mrc1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mrc1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CD206/CLEC13D ELISA Kit PicoKine® (EK2050)
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Customer Q&As
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6 Customer Q&As for Mouse CD206/CLEC13D ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CD206 ELISA kit? I performed your CD206 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD206 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-08-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD206 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of CD206 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-08-24
Question
Q: can I use EDTA plasma as samples in Mouse CD206 Picokine® ELISA Kit (Catalog # EK2050)?
W. Bhatt
Verified customer
Asked: 2019-10-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CD206 causing disruption of its protein structure. CD206 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD206 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-15
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD206 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
S. Wright
Verified customer
Asked: 2018-10-23
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD206 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-23
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-02-07
Question
Q: if the enzyme conjugated CD206 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD206 antigen?
R. Miller
Verified customer
Asked: 2017-03-08
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-03-08
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD206 using the EK2050 Mouse CD206 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-07-20
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2016-07-20
