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Product Info Summary
| SKU: | EK0313 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | bone tissue, cell culture supernatants and serum. |
Product info
Product Name
Mouse BMP-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0313
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse BMP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Bmp2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse BMP-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0313)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q283-R396
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0313 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0313 is reactive to Bmp2 in Mouse samples
Validated Sample Types
bone tissue, cell culture supernatants and serum.
Application Guarantee
EK0313 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BMP2
Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0313-CAP | Anti-Mouse Bmp2 Pre-coated 96-well strip microplate | 1 |
| EK0313-ST | Mouse Bmp2 Standard | 2 vials, 10 ng/tube |
| EK0313-DA | Mouse Bmp2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.002 | 0.016 | 0.027 | 0.056 | 0.179 | 0.415 | 1.118 | 2.016 |
Data Example Images
Click image to see more details
Mouse BMP-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BMP2, Dilution ratio of 1:1, concentration in serum is around 1 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse BMP-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 62 | 249 | 770 | 61 | 243 | 710 |
| Standard deviation | 2.54 | 10.95 | 58.52 | 2.62 | 12.63 | 55.38 |
| CV (%) | 4.1% | 4.4% | 7.6% | 4.3% | 5.2% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BMP2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 62 | 70 | 61 | 71 | 66 | 4.52 | 6.8% |
| Sample 2 | 249 | 252 | 246 | 234 | 245 | 6.83 | 2.7% |
| Sample 3 | 770 | 853 | 881 | 850 | 838 | 41.35 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For Bmp2 (Source: Uniprot.Org, NCBI)
Gene Name
Bmp2
Full Name
Bone morphogenetic protein 2
Weight
44525 MW
Superfamily
TGF-beta family
Alternative Names
Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;Bmp2;Bmp-2; BMP2 BDA2A, SSFSC, SSFSC1, BMP2 bone morphogenetic protein 2 bone morphogenetic protein 2|bone morphogenetic protein 2A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Bmp2, check out the Bmp2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Bmp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse BMP-2 ELISA Kit PicoKine® (EK0313)
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Mouse BMP-2 ELISA Kit PicoKine®
Question
Q: Can BMP2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
V. Lee
Verified customer
Asked: 2020-09-27
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-09-27
Question
Q: What is the optimal O.D. value for BMP2 ELISA kit? I performed your BMP2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain BMP2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-05-29
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP2 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of BMP2 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2020-05-29
Question
Would the mouse antibody in this kit cross-react with the rat sample?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
BMP-2 is conservative and shows high homology in human, mouse and rat. Therefore, our mouse antibody in this kit should cross-react with the rat sample.
Boster Scientific Support
Answered: 2020-03-24
Question
Q: can you suggest the dilution ratio of serum samples for detection of BMP2 in Mouse bone tissue? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
S. Rodriguez
Verified customer
Asked: 2020-02-18
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the BMP2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-02-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Evans
Verified customer
Asked: 2020-02-01
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-02-01
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-09-17
Question
Are the antibody pairs in the EZ0313 same as the one used in the EK0313 or FEK0313 kit?
Verified customer
Asked: 2019-04-10
Answer
The antibody pairs in the Mouse BMP-2 EZ-Set ELISA Kit (DIY Antibody Pairs) (EZ0313) are different from the one used in the EK0313 or FEK0313 kit.
Boster Scientific Support
Answered: 2019-04-15
Question
Have you tested EK0313 with mouse plasma and what is the dilution ratio you used?
Verified customer
Asked: 2019-04-09
Answer
For the Mouse BMP-2 ELISA Kit PicoKine™ (EK0313), the suggested dilution ratio of mouse plasma is 1:1.
Boster Scientific Support
Answered: 2019-04-10
Question
Q: if the enzyme conjugated BMP2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP2 antigen?
Verified Customer
Verified customer
Asked: 2019-01-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-01-06
Question
Q: how to thaw whole blood sample for BMP2 ELISA after freezing?
M. White
Verified customer
Asked: 2015-08-15
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2015-08-15
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
D. Phillips
Verified customer
Asked: 2015-03-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-03-14
Question
Hi, I am interested in your Mouse BMP-2 PicoKine
Verified Customer
Verified customer
Asked: 2014-03-12
Answer
The suggested dilution ratio of mouse plasma is 1:1.
Boster Scientific Support
Answered: 2014-03-12
