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Product Info Summary
| SKU: | EK0311-EQ |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Equine |
| Application: | ELISA |
| Sample Types: | bone tissue, cell culture supernatants and serum. |
Product info
Product Name
Horse equine BMP-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0311-EQ
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Horse equine BMP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Horse BMP2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Horse equine BMP-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0311-EQ)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q283-R396
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0311-EQ for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0311-EQ is reactive to BMP2 in Equine samples
Validated Sample Types
bone tissue, cell culture supernatants and serum.
Application Guarantee
EK0311-EQ is guaranteed for ELISA in Equine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BMP2
Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant horse equine BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0311-EQ-CAP | Anti-Equine BMP2 Pre-coated 96-well strip microplate | 1 |
| EK0311-EQ-ST | Equine BMP2 Standard | 2 vials, 10 ng/tube |
| EK0311-EQ-DA | Equine BMP2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.005 | 0.034 | 0.059 | 0.119 | 0.277 | 0.68 | 1.642 | 2.417 |
Data Example Images
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Horse equine BMP-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Horse equine BMP-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 285 | 742 | 1197 | 306 | 834 | 1358 |
| Standard deviation | 9.12 | 23.17 | 37.12 | 18.97 | 58.38 | 97.78 |
| CV (%) | 3.2 | 3.5 | 3.1 | 6.2 | 7 | 7.2 |
Protein Target Info & Infographic
Gene/Protein Information For BMP2 (Source: Uniprot.Org, NCBI)
Gene Name
BMP2
Full Name
Bone morphogenetic protein 2
Weight
44702 MW
Superfamily
TGF-beta family
Alternative Names
Bone morphogenetic protein 2 ;BMP2 ; BMP2 BDA2A, SSFSC, SSFSC1, BMP2 bone morphogenetic protein 2 bone morphogenetic protein 2|bone morphogenetic protein 2A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BMP2, check out the BMP2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Horse equine BMP-2 ELISA Kit PicoKine® (EK0311-EQ)
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Customer Q&As
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6 Customer Q&As for Horse equine BMP-2 ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
S. Mitchell
Verified customer
Asked: 2020-12-12
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-12-12
Question
Q: how to thaw whole blood sample for BMP2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-05-27
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-05-27
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-07-19
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-10-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-10-06
Question
Q: What is the optimal O.D. value for BMP2 ELISA kit? I used your BMP2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain BMP2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-09-29
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP2 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of BMP2 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2018-09-29
Question
Q: if the enzyme conjugated BMP2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP2 antigen?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-12-29
