Monkey primate TIMP-3 ELISA Kit PicoKine®

TIMP-3 ELISA kit for Monkey

Monkey primate TIMP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Monkey TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK0523-PR
Size: 96 wells/kit, with removable strips.
Reactive Species: Monkey
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Product Name

Monkey primate TIMP-3 ELISA Kit PicoKine®

View all TIMP-3 ELISA kits

SKU/Catalog Number

EK0523-PR

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Monkey primate TIMP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Monkey TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Monkey primate TIMP-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0523-PR)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: C24-P211

Sensitivity

<2 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK0523-PR for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0523-PR is reactive to TIMP3 in Monkey samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EK0523-PR is guaranteed for ELISA in Monkey by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TIMP-3

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).

Kit Components

Catalog Number Description Quantity
EK0523-PR-CAP Anti-Monkey TIMP3 Pre-coated 96-well strip microplate 1
EK0523-PR-ST Monkey TIMP3 Standard 2 vials, 10 ng/tube
EK0523-PR-DA Monkey TIMP3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0080.0520.0790.1600.3560.8261.6932.485

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Monkey primate TIMP-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)125026875353143028325657
Standard deviation72.5110.2192.795.81147.3277.2
CV (%)5.84.13.66.75.24.9

Gene/Protein Information For TIMP3 (Source: Uniprot.Org, NCBI)

Gene Name

TIMP3

Full Name

Metalloproteinase inhibitor 3

Weight

24145 MW

Superfamily

protease inhibitor I35 (TIMP) family

Alternative Names

Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3; TIMP3 HSMRK222, K222, K222TA2, SFD TIMP metallopeptidase inhibitor 3 metalloproteinase inhibitor 3|MIG-5 protein|TIMP-3|protein MIG-5|tissue inhibitor of metalloproteinases 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TIMP3, check out the TIMP3 Infographic

TIMP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TIMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EK0523-PR

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Monkey primate TIMP-3 ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Monkey primate TIMP-3 ELISA Kit PicoKine®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Monkey primate TIMP-3 ELISA Kit PicoKine®

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-06-10

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-06-10

Question

Q: which procedure should I follow in order to thaw whole blood sample for TIMP-3 ELISA after freezing?

P. Kulkarni

Verified customer

Asked: 2019-01-06

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TIMP-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-01-06

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TIMP-3 in Monkey plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-02-27

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TIMP-3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-02-27

Question

Q: if the enzyme conjugated TIMP-3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TIMP-3 antigen?

Verified Customer

Verified customer

Asked: 2017-04-26

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-04-26

Question

Q: can I use citrate plasma as samples in Monkey TIMP-3 Picokine® ELISA Kit (Catalog # EK0523-PR)?

Verified Customer

Verified customer

Asked: 2016-11-27

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TIMP-3 causing degradation of its protein structure. TIMP-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the TIMP-3 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-11-27

Question

Q: how to analyze ELISA data? I have obtained TIMP-3 level in plasma.

Verified Customer

Verified customer

Asked: 2016-11-14

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-11-14

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

T. Jha

Verified customer

Asked: 2014-11-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2014-11-16

$499
Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
In stock
Order Product
EK0523-PR
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.