Product Info Summary
SKU: | FEK0523 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum, plasma (heparin , EDTA) and saliva |
Assay Time: | 90 Minutes |
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Product info
Product Name
Human TIMP3 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0523
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TIMP3 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin , EDTA) and saliva. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TIMP3 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0523)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: C24-P211
Sensitivity
<2 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0523 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0523 is reactive to TIMP3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin , EDTA) and saliva
Application Guarantee
FEK0523 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TIMP-3
The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).
Kit Components
Catalog number | Description | Quantity | Volume |
---|---|---|---|
FEK0523-CAP | Anti-Human TIMP-3 Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
EK0523-ST | Human TIMP-3 Standard | 2 | 10 ng/tube |
FEK0523-H | HRP Linked Anti-Human TIMP3 Antibody | 1 | 6ml |
AR1106-1 | Sample Diluent | 1 | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
AR1105 | Stop Solution | 1 | 10ml |
PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.111 | 0.160 | 0.190 | 0.231 | 0.414 | 1.003 | 2.177 | 2.388 |
Data Example Images
![fek0523 fek0523](https://www.bosterbio.com/media/catalog/product/f/e/fek0523.png)
Click image to see more details
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 228 | 1364 | 4394 | 244 | 1487 | 4066 |
Standard deviation | 14.59 | 90.02 | 237.27 | 19.03 | 107.06 | 333.41 |
CV (%) | 6.4% | 6.6% | 5.4% | 7.8% | 7.2% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TIMP-3 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 228 | 257 | 260 | 256 | 250 | 11.25 | 4.5% |
Sample 2 | 1364 | 1309 | 1336 | 1522 | 1382 | 81.53 | 5.9% |
Sample 3 | 4394 | 4601 | 4288 | 4589 | 4468 | 285.95 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For TIMP3 (Source: Uniprot.Org, NCBI)
Gene Name
TIMP3
Full Name
Metalloproteinase inhibitor 3
Weight
Superfamily
protease inhibitor I35 (TIMP) family
Alternative Names
HSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; pseudoinflammatory); SFD; TIMP metallopeptidase inhibitor 3; TIMP3; TIMP-3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy; Tissue inhibitor of metalloproteinases 3 TIMP3 HSMRK222, K222, K222TA2, SFD TIMP metallopeptidase inhibitor 3 metalloproteinase inhibitor 3|MIG-5 protein|TIMP-3|protein MIG-5|tissue inhibitor of metalloproteinases 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TIMP3, check out the TIMP3 Infographic
![TIMP3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TIMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TIMP3 PicoKine® Quick ELISA Kit (FEK0523)
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