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Product Info Summary
| SKU: | EK0523-PO |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Pig |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
Product info
Product Name
Pig porcine TIMP-3 ELISA Kit PicoKine®
SKU/Catalog Number
EK0523-PO
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Pig porcine TIMP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Pig TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Pig porcine TIMP-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0523-PO)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: C24-P211
Sensitivity
<2 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0523-PO for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0523-PO is reactive to TIMP3 in Pig samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
Application Guarantee
EK0523-PO is guaranteed for ELISA in Pig by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TIMP-3
The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0523-PO-CAP | Anti-Pig TIMP3 Pre-coated 96-well strip microplate | 1 |
| EK0523-PO-ST | Pig TIMP3 Standard | 2 vials, 10 ng/tube |
| EK0523-PO-DA | Pig TIMP3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.019 | 0.080 | 0.118 | 0.219 | 0.437 | 0.808 | 1.571 | 2.420 |
Data Example Images
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Pig TIMP-3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Pig porcine TIMP-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1250 | 2687 | 5353 | 1430 | 2832 | 5657 |
| Standard deviation | 72.5 | 110.2 | 192.7 | 95.81 | 147.3 | 277.2 |
| CV (%) | 5.8% | 4.1% | 3.6% | 6.7% | 5.2% | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For TIMP3 (Source: Uniprot.Org, NCBI)
Gene Name
TIMP3
Full Name
Metalloproteinase inhibitor 3
Weight
24145 MW
Superfamily
protease inhibitor I35 (TIMP) family
Alternative Names
Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3; TIMP3 HSMRK222, K222, K222TA2, SFD TIMP metallopeptidase inhibitor 3 metalloproteinase inhibitor 3|MIG-5 protein|TIMP-3|protein MIG-5|tissue inhibitor of metalloproteinases 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TIMP3, check out the TIMP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TIMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Pig porcine TIMP-3 ELISA Kit PicoKine® (EK0523-PO)
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Customer Q&As
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9 Customer Q&As for Pig porcine TIMP-3 ELISA Kit PicoKine®
Question
Q: which procedure should I follow in order to thaw whole blood sample for TIMP-3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-11-30
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TIMP-3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-11-30
Question
Q: can I use citrate plasma as samples in Pig TIMP-3 Picokine® ELISA Kit (Catalog # EK0523-PO)?
K. Gonzalez
Verified customer
Asked: 2020-11-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TIMP-3 causing disruption of its protein structure. TIMP-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TIMP-3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-11-28
Question
Q: if the enzyme conjugated TIMP-3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TIMP-3 antigen?
W. Liu
Verified customer
Asked: 2019-07-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-07-29
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-07-22
Question
Q: how to analyze ELISA data? I measured TIMP-3 level in serum.
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-06-10
Question
Q: What is the optimal O.D. value for TIMP-3 ELISA kit? I performed your TIMP-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TIMP-3 even though the O.D. values are not very high?
K. Smith
Verified customer
Asked: 2018-12-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TIMP-3 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of TIMP-3 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2018-12-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-09-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Brown
Verified customer
Asked: 2018-05-10
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-05-10
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-10-13
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-10-13
