Product Info Summary
SKU: | PROTP80075 |
---|---|
Size: | 2ug, 10ug, 1mg |
Origin Species: | Human |
Source: | Escherichia coli |
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Product info
Product Name
MCP-2 Monocyte Chemotactic Protein-2 Human Recombinant Protein (CCL8)
View all CCL8/MCP-2 recombinant proteins
SKU/Catalog Number
PROTP80075
Size
2ug, 10ug, 1mg
Description
Monocyte Chemotactic Protein-2 Human Recombinant produced in E. coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton. The MCP2 is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized MCP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL8should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Cite This Product
MCP-2 Monocyte Chemotactic Protein-2 Human Recombinant Protein (CCL8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP80075)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Reconstitution
It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-2in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.
Biological Activity
The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ng/mL corresponding to a Specific Activity of 10,000-33,334IU/mg.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-2in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
![recombinant protein thumbnail_1 recombinant protein thumbnail_1](https://www.bosterbio.com/media/catalog/product/r/e/recombinant-protein-thumbnail_1.png)
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For CCL8 (Source: Uniprot.org, NCBI)
Gene Name
CCL8
Full Name
C-C motif chemokine 8
Weight
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8 CCL8 HC14, MCP-2, MCP2, SCYA10, SCYA8 C-C motif chemokine ligand 8 C-C motif chemokine 8|chemokine (C-C motif) ligand 8|monocyte chemoattractant protein 2|monocyte chemotactic protein 2|small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)|small-inducible cytokine A8
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CCL8, check out the CCL8 Infographic
![CCL8 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCL8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For MCP-2 Monocyte Chemotactic Protein-2 Human Recombinant Protein (CCL8) (PROTP80075)
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