MCP-2 Monocyte Chemotactic Protein-2 Human Recombinant Protein (CCL8)

Product Name

MCP-2 Monocyte Chemotactic Protein-2 Human Recombinant Protein (CCL8)

View all CCL8/MCP-2 recombinant proteins

SKU/Catalog Number

PROTP80075

Size

2ug, 10ug, 1mg

Description

Monocyte Chemotactic Protein-2 Human Recombinant produced in E. coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton. The MCP2 is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized MCP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL8should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

MCP-2 Monocyte Chemotactic Protein-2 Human Recombinant Protein (CCL8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP80075)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

11.246kDa

Reconstitution

It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-2in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ng/mL corresponding to a Specific Activity of 10,000-33,334IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-2in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For CCL8 (Source: Uniprot.org, NCBI)

Alternative Names

Small inducible cytokine A8; CCL8; Monocyte chemotactic protein 2; MCP-2; Monocyte chemoattractant protein 2; HC14; chemokine (C-C motif) ligand 8; MCP2; SCYA8; SCYA10 CCL8 HC14, MCP-2, MCP2, SCYA10, SCYA8 C-C motif chemokine ligand 8 C-C motif chemokine 8|chemokine (C-C motif) ligand 8|monocyte chemoattractant protein 2|monocyte chemotactic protein 2|small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)|small-inducible cytokine A8

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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